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J Biol Chem

Title:		Genetic evidence for a tyrosine kinase cascade preceding the mitogen-activated protein kinase cascade in vertebrate G protein signaling
Author(s):		Wan Y; Bence K; Hata A; Kurosaki T; Veillette A; Huang XY;
Address:		"Department of Physiology, Cornell University Medical College, New York, New York 10021, USA"
Journal Title:		J Biol Chem
Year:		1997
Volume:		272
Issue:		27
Page Number:		17209 - 17215
DOI:		10.1074/jbc.272.27.17209
ISSN/ISBN:		0021-9258 (Print) 0021-9258 (Linking)
Abstract:		"The signal transduction pathway from heterotrimeric G proteins to the mitogen-activated protein kinase (MAPK) cascade is best understood in the yeast mating pheromone response, in which a serine/threonine protein kinase (STE20) serves as the critical linking component. Little is known in metazoans on how G proteins and the MAPK cascade are coupled. Here we provide genetic and biochemical evidence that a tyrosine kinase cascade bridges G proteins and the MAPK pathway in vertebrate cells. Targeted deletion of tyrosine kinase Csk in avian B lymphoma cells blocks the stimulation of MAPK by Gq-, but not Gi-, coupled receptors. In cells deficient in Bruton's tyrosine kinase (Btk), Gi-coupled receptors failed to activate MAPK, while Gq-coupled receptor-mediated stimulation is unaffected. Taken together with our previous data on tyrosine kinases Lyn and Syk, the Gq-coupled pathway requires tyrosine kinases Csk, Lyn, and Syk, while the Gi-coupled pathway requires tyrosine kinases Btk and Syk to feed into the MAPK cascade in these cells. The central role of Syk is further strengthened by data showing that Syk can bind to purified Lyn, Csk, or Btk"
Keywords:		Agammaglobulinaemia Tyrosine Kinase Animals Binding Sites CSK Tyrosine-Protein Kinase Calcium-Calmodulin-Dependent Protein Kinases/*genetics/metabolism Enzyme Precursors/metabolism GTP-Binding Proteins/*genetics/metabolism Intracellular Signaling Peptides;
Notes:		"MedlineWan, Y Bence, K Hata, A Kurosaki, T Veillette, A Huang, X Y eng Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S. 1997/07/04 J Biol Chem. 1997 Jul 4; 272(27):17209-15. doi: 10.1074/jbc.272.27.17209"