| Title: | Importance of enhanced mass resolution in removing interferences when measuring volatile organic compounds in human blood by using purge-and-trap gas chromatography/mass spectrometry |
| Author(s): | Bonin MA; Ashley DL; Cardinali FL; McGraw JM; Patterson DG; |
| Address: | "Division of Environmental Health Laboratory Sciences, National Center for Environmental Health and Injury Control, Centers for Disease Control, Public Health Service, U.S. Department of Health and Human Services, F 17, 1600 Clifton Road, 30333, Atlanta, Georgia, USA" |
| DOI: | 10.1016/1044-0305(92)80006-7 |
| ISSN/ISBN: | 1044-0305 (Print) 1044-0305 (Linking) |
| Abstract: | "The number of volatile organic compounds (VOCs) that can be purged from human blood is so great that they cannot be separated completely by capillary gas chromatography. As a result, the single-mass chromatograms used for quantitating the target compounds by mass spectrometry have many interferences at nominal (integer) mass resolution of a quadrupole mass spectrometer. The results of these interferences range from small errors in quantitation to completely erroneous results for the target VOCs. By using a magnetic sector mass spectrometer, these interferences at nominal mass can be removed at higher resolution by lowering the ion chromatogram windows around the masses of interest. At 3000 resolution (10% valley definition), unique single-ion chromatograms can be made for the quantitation ions of the target VOCs. Full-scan mass data are required to allow the identification of unknown compounds purged from the blood. By using isotope-dilution mass spectrometry, most target VOCs can be detected in the low parts per trillion range for a 10-mL quantity of blood from which the VOCs have been removed by a purge-and-trap method" |
| Notes: | "PubMed-not-MEDLINEBonin, M A Ashley, D L Cardinali, F L McGraw, J M Patterson, D G eng 1992/11/01 J Am Soc Mass Spectrom. 1992 Nov; 3(8):831-41. doi: 10.1016/1044-0305(92)80006-7" |
