| Title: | "Data-driven design of a multiplexed, peptide-sensitized transistor to detect breath VOC markers of COVID-19" |
| Author(s): | Nakano-Baker O; Fong H; Shukla S; Lee RV; Cai L; Godin D; Hennig T; Rath S; Novosselov I; Dogan S; Sarikaya M; MacKenzie JD; |
| Address: | "University of Washington Dept. of Materials Science and Engineering, USA. Electronic address: onb@uw.edu. University of Washington Dept. of Materials Science and Engineering, USA. University of Washington Dept. of Biochemistry, USA. University of Washington Dept. of Atmospheric Chemistry, USA. University of Washington Depts. of Mechanical Engineering, Occupational and Environmental Health Sciences, USA. University of Washington School of Dentistry, USA. University of Washington Depts. of Materials Science and Engineering, Chemical Engineering, Oral Health Sciences, USA. University of Washington Depts. of Materials Science and Engineering, Mechanical Engineering, USA" |
| DOI: | 10.1016/j.bios.2023.115237 |
| ISSN/ISBN: | 1873-4235 (Electronic) 0956-5663 (Print) 0956-5663 (Linking) |
| Abstract: | "Exhaled human breath contains a rich mixture of volatile organic compounds (VOCs) whose concentration can vary in response to disease or other stressors. Using simulated odorant-binding proteins (OBPs) and machine learning methods, we designed a multiplex of short VOC- and carbon-binding peptide probes that detect a characteristic 'VOC fingerprint'. Specifically, we target VOCs associated with COVID-19 in a compact, molecular sensor array that directly transduces vapor composition into multi-channel electrical signals. Rapidly synthesizable, chimeric VOC- and solid-binding peptides were derived from selected OBPs using multi-sequence alignment with protein database structures. Selective peptide binding to targeted VOCs and sensor surfaces was validated using surface plasmon resonance spectroscopy and quartz crystal microbalance. VOC sensing was demonstrated by peptide-sensitized, exposed-channel carbon nanotube transistors. The data-to-device pipeline enables the development of novel devices for non-invasive monitoring, diagnostics of diseases, and environmental exposure assessment" |
| Keywords: | Humans *Biosensing Techniques *COVID-19/diagnosis *Volatile Organic Compounds/chemistry Environmental Exposure Surface Plasmon Resonance Breath Tests/methods Carbon nanotube transistor Molecular design Multiplex biosensors Odorant binding proteins Solid-b; |
| Notes: | "MedlineNakano-Baker, Oliver Fong, Hanson Shukla, Shalabh Lee, Richard V Cai, Le Godin, Dennis Hennig, Tatum Rath, Siddharth Novosselov, Igor Dogan, Sami Sarikaya, Mehmet MacKenzie, J Devin eng U01 HL152401/HL/NHLBI NIH HHS/ England 2023/03/26 Biosens Bioelectron. 2023 Jun 1; 229:115237. doi: 10.1016/j.bios.2023.115237. Epub 2023 Mar 20" |
