« Previous AbstractMolecular characterization and expression of sensory neuron membrane proteins in the parasitoid Microplitis mediator (Hymenoptera: Braconidae)    Next AbstractDependence of volatile droplet lifetime on the hydrophobicity of the substrate »

J Adv Res

Title:		A female-biased odorant receptor tuned to the lepidopteran sex pheromone in parasitoid Microplitis mediator guiding habitat of host insects
Author(s):		Shan S; Song X; Khashaveh A; Wang SN; Lu ZY; Hussain Dhiloo K; Li RJ; Zhang YJ;
Address:		"State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China. College of Plant Protection, Agricultural University of Hebei, Baoding 071000, China; College of Plant Protection, China Agricultural University, Beijing 100193, China. Institute of Plant and Environment Protection, Beijing Academy of Agricultural and Forestry Sciences, Beijing 100097, China. IPM Center of Hebei Province, Key Laboratory of Integrated Pest Management on Crops in Northern Region of North China, Ministry of Agriculture, Plant Protection Institute, Hebei Academy of Agricultural and Forestry Sciences, Baoding 071000, China. Department of Entomology, Faculty of Crop Protection, Sindh Agriculture University, Tandojam 70060, Pakistan. College of Plant Protection, Agricultural University of Hebei, Baoding 071000, China. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China. Electronic address: yjzhang@ippcaas.cn"
Journal Title:		J Adv Res
Year:		2023
Volume:		20220307
Issue:
Page Number:		1 - 12
DOI:		10.1016/j.jare.2022.03.006
ISSN/ISBN:		2090-1224 (Electronic) 2090-1232 (Print) 2090-1224 (Linking)
Abstract:		"INTRODUCTION: The parasitoid wasp Microplitis mediator is an important natural enemy of the turnip moth Agrotis segetum and other Noctuidae pests. In our field observation, it was fortuitously discovered that sex pheromone traps used for A. segetum also attract female wasps, verified by a simulated field condition dual-choice laboratory assay. Therefore, it was hypothesized that olfactory recognition could be crucial in this process. In this regard, a female-biased odorant receptor of the wasp, MmedOR49, attracted our attention. OBJECTIVES: To unravel the significance of the female-biased MmedOR49 regulating host pheromone recognition. METHODS: Expression analysis (fluorescence in situ hybridization; quantitative realtime PCR), in vitro (two-electrode voltage-clamp recordings) and in vivo (RNAi combined with behavioral assessments) functional studies, and bioinformatics (structural modeling and molecular docking) were carried out to investigate the characteristics of MmedOR49. RESULTS: MmedOR49 expression was detected in the antennae of females by FISH. Quantification indicated that the expression level of MmedOR49 increased significantly after adult emergence. In vitro functional study revealed that MmedOR49 was specifically tuned to cis-5-decenyl acetate (Z5-10:Ac), the major sex pheromone component of A. segetum. Molecular docking showed that Z5-10:Ac strongly bound to the key amino acid residues His 80, Ile 81, and Arg 84 of MmedOR49 through hydrogen bonding. Behavioral assays indicated that female wasps were significantly attracted by Z5-10:Ac in a three-cage olfactometer. RNAi targeting further confirmed that MmedOR49 was necessary to recognize Z5-10:Ac, as female wasps lost their original behavioral responses to Z5-10:Ac after down-regulation of the MmedOR49 transcript. CONCLUSION: Although M. mediator is a larval endoparasitoid, female wasps have a behavioral preference for a sex pheromone component of lepidopteran hosts. In this behavior, for female M. mediator, MmedOR49 plays an important role in guiding the habitat of host insects. These data provide a potential target for enhancing natural enemy utilization and pest control"
Keywords:		"Female Animals *Receptors, Odorant/genetics/metabolism *Sex Attractants/metabolism In Situ Hybridization, Fluorescence Molecular Docking Simulation *Wasps/genetics/metabolism *Moths/genetics/metabolism Host habitat location Lepidopteran pest Microplitis m;"
Notes:		"MedlineShan, Shuang Song, Xuan Khashaveh, Adel Wang, Shan-Ning Lu, Zi-Yun Hussain Dhiloo, Khalid Li, Rui-Jun Zhang, Yong-Jun eng Research Support, Non-U.S. Gov't Egypt 2022/12/31 J Adv Res. 2023 Jan; 43:1-12. doi: 10.1016/j.jare.2022.03.006. Epub 2022 Mar 7"