| Title: | Molecular characterization and expression of sensory neuron membrane proteins in the parasitoid Microplitis mediator (Hymenoptera: Braconidae) |
| Author(s): | Shan S; Wang SN; Song X; Khashaveh A; Lu ZY; Dhiloo KH; Li RJ; Gao XW; Zhang YJ; |
| Address: | "College of Plant Protection, China Agricultural University, Beijing, China. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China. Institute of Plant and Environment Protection, Beijing Academy of Agricultural and Forestry Sciences, Beijing, China. College of Plant Protection, Agricultural University of Hebei, Baoding, China. IPM Center of Hebei Province, Key Laboratory of Integrated Pest Management on Crops in Northern Region of North China, Ministry of Agriculture, Plant Protection Institute, Hebei Academy of Agricultural and Forestry Sciences, Baoding, China. Department of Entomology, Faculty of Crop Protection, Sindh Agriculture University, Tandojam, Pakistan" |
| ISSN/ISBN: | 1744-7917 (Electronic) 1672-9609 (Print) 1672-9609 (Linking) |
| Abstract: | "Sensory neuron membrane proteins (SNMPs), homologs of the human fatty acid transport protein CD36 family, are observed to play a significant role in chemoreception, especially in detecting sex pheromone in Drosophila and some lepidopteran species. In the current study, two full-length SNMP transcripts, MmedSNMP1 and MmedSNMP2, were identified in the parasitoid Microplitis mediator (Hymenoptera: Braconidae). Quantitative real-time polymerase chain reaction analysis showed that the expression of MmedSNMP1 was significantly higher in antennae than in other tissues of both sexes. In addition, the MmedSNMP1 transcript was increased dramatically in newly emerged adults and there were no significant differences between adults with or without mating and parasitic experiences. However, compared with MmedSNMP1, the expression of MmedSNMP2 was widely found in various tissues, significantly increased at half-pigmented pupae stage and remained at a relatively constant level during the following developmental stages. It was found that MmedSNMP1 contained eight exons and seven introns, which was highly conserved compared with other insect species. In situ hybridization assay demonstrated that MmedSNMP1 transcript was distributed widely in antennal flagella. Among selected chemosensory genes (odorant binding protein, odorant receptor, and ionotropic receptor genes), MmedSNMP1 only partially overlapped with MmedORco in olfactory sensory neurons of antennae. Subsequent immunolocalization results further indicated that MmedSNMP1 was mainly expressed in sensilla placodea of antennae and possibly involved in perceiving plant volatiles and sex pheromones. These findings lay a foundation for further investigating the roles of SNMPs in the chemosensation of parasitoids" |
| Keywords: | "Animals Arthropod Antennae/metabolism Drosophila Proteins/genetics/*metabolism In Situ Hybridization Insect Proteins/genetics/metabolism Membrane Proteins/genetics/metabolism Parasites/metabolism/physiology Phylogeny Receptors, Odorant/genetics/*metabolis;" |
| Notes: | "MedlineShan, Shuang Wang, Shan-Ning Song, Xuan Khashaveh, Adel Lu, Zi-Yun Dhiloo, Khalid Hussain Li, Rui-Jun Gao, Xi-Wu Zhang, Yong-Jun eng 31471778/National Natural Science Foundation of China/ 31621064/National Natural Science Foundation of China/ 31672038/National Natural Science Foundation of China/ 31772176/National Natural Science Foundation of China/ Australia 2019/02/20 Insect Sci. 2020 Jun; 27(3):425-439. doi: 10.1111/1744-7917.12667. Epub 2019 Mar 14" |
