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J Chromatogr B Analyt Technol Biomed Life Sci

Title:		"A validated LC-MS/MS method for thyroid hormone determination in sea lamprey (Petromyzon marinus) plasma, gill, kidney and liver"
Author(s):		Bussy U; Chung-Davidson YW; Li K; Fissette SD; Buchinger EG; Li W;
Address:		"Department of Fisheries and Wildlife, Michigan State University, Room 13 Natural Resources Building, 480 Wilson Road, East Lansing, MI 48824, USA. Electronic address: ugo.bussy@gmail.com. Department of Fisheries and Wildlife, Michigan State University, Room 13 Natural Resources Building, 480 Wilson Road, East Lansing, MI 48824, USA. Department of Fisheries and Wildlife, Michigan State University, Room 13 Natural Resources Building, 480 Wilson Road, East Lansing, MI 48824, USA. Electronic address: liweim@msu.edu"
Journal Title:		J Chromatogr B Analyt Technol Biomed Life Sci
Year:		2017
Volume:		20161219
Issue:
Page Number:		77 - 84
DOI:		10.1016/j.jchromb.2016.12.024
ISSN/ISBN:		1873-376X (Electronic) 1570-0232 (Linking)
Abstract:		"A UPLC-MS/MS method was developed to provide a reproducible, sensitive and quantitative assay to determine thyroid hormones in sea lamprey tissues and plasma. l-Thyroxine (T4) and its two triiodo-thyronine isomers have been simultaneously quantified and validated for plasma, gill, liver, and kidney matrices. Multiple sample preparation techniques were investigated to achieve optimal sample matrix digestion and clean-up. Enzymatic digestion followed by protein precipitation was selected to process the samples. The developed method exhibited excellent linearity for all analytes with regression coefficients higher than 0.99 for concentrations ranged from 10 to 50,000pg/mL. The limit of detection (LOD) was under 1pg/mL while the limit of quantification (LOQ) was estimated as 10pg/mL. This method was validated according to the FDA guidance and applied to determine thyroid hormone levels in plasma, gill and kidney of sea lamprey exposed to a sex pheromone. With appropriate implementation and further validation, this method could be applied to tissues in other species including humans"
Keywords:		"Animals Chromatography, Liquid/*methods Gills/chemistry Kidney/chemistry Limit of Detection Linear Models Liver/chemistry Petromyzon/*metabolism Reproducibility of Results Tandem Mass Spectrometry/*methods Thyroid Hormones/*analysis/metabolism Tissue Dist;"
Notes:		"MedlineBussy, Ugo Chung-Davidson, Yu-Wen Li, Ke Fissette, Skye D Buchinger, Ethan G Li, Weiming eng Netherlands 2016/12/25 J Chromatogr B Analyt Technol Biomed Life Sci. 2017 Jan 15; 1041-1042:77-84. doi: 10.1016/j.jchromb.2016.12.024. Epub 2016 Dec 19"