| Title: | Expression of functional Raphanus sativus antifungal protein in yeast |
| Author(s): | Alves AL; De Samblanx GW; Terras FR; Cammue BP; Broekaert WF; |
| Address: | "F.A. Janssens Laboratory of Genetics, Catholic University of Leuven, Heverlee, Belgium" |
| DOI: | 10.1016/0014-5793(94)00597-4 |
| ISSN/ISBN: | 0014-5793 (Print) 0014-5793 (Linking) |
| Abstract: | Rs-AFP2 is a 51 amino acid cysteine-rich peptide isolated from radish (Raphanus sativus) seeds that exhibits potent inhibitory activity against filamentous fungi. A cDNA clone encoding the Rs-AFP2 preprotein was modified by recombinant DNA methods to allow expression in the yeast Saccharomyces cerevisiae. This peptide was expressed in yeast as a fusion protein carrying at its N-terminus the prepro-sequences derived from the precursor of the yeast pheromone mating factor alpha 1. These sequences allow secretion of the biologically active peptide in a correctly processed form. Deletion of the mating factor alpha 1 pro-peptide drastically reduced the expression level of the peptide |
| Keywords: | Amino Acid Sequence *Antifungal Agents Base Sequence Fusarium/drug effects *Gene Expression Gene Transfer Techniques Genetic Vectors Molecular Sequence Data Plant Proteins/chemistry/*genetics/pharmacology Plasmids Polymerase Chain Reaction Recombinant Fus; |
| Notes: | "MedlineAlves, A L De Samblanx, G W Terras, F R Cammue, B P Broekaert, W F eng Research Support, Non-U.S. Gov't England 1994/07/18 FEBS Lett. 1994 Jul 18; 348(3):228-32. doi: 10.1016/0014-5793(94)00597-4" |
