« Previous Abstract"Traffic related air pollution and acute hospital admission for respiratory diseases in Drammen, Norway 1995-2000"    Next AbstractVolatile organic compounds: A proinflammatory activator in autoimmune diseases »

Yeast

Title:		Regulation of mating and mating-type-specific genes in Zygosaccharomyces sp. yeast
Author(s):		Ogata T; Kuroki K;
Address:		"Department of Biotechnology, Maebashi Institute of Technology, Maebashi, Japan"
Journal Title:		Yeast
Year:		2021
Volume:		20210409
Issue:		8
Page Number:		471 - 479
DOI:		10.1002/yea.3561
ISSN/ISBN:		1097-0061 (Electronic) 0749-503X (Linking)
Abstract:		"Zygosaccharomyces sp. is an industrially important yeast for the production traditional fermented foods in Japan. At present, however, there is no easy method for mating Zygosaccharomyces sp. strains in the laboratory; furthermore, little is known about the expression of mating-type-specific genes in this yeast. Here, mating was observed when Zygosaccharomyces sp. was subjected to nitrogen-starvation conditions. The expression of mating-type-specific genes, Zygo STE6 and Zygo MFalpha1, was induced under nitrogen-starvation conditions, as confirmed by lacZ reporter assay. This expression was mating-type-specific: Zygo STE6 was expressed specifically for mating-type a, whereas and Zygo MFalpha1 was expressed specifically for mating-type alpha. Yeast strains Zygosaccharomyces rouxii DL2 and DA2, derived from type strain Z. rouxii CBS732, did not show mating even under nitrogen-starvation conditions. Gene sequencing revealed that the Zygo STE12 in Z. rouxii CBS732 has a frameshift mutation. Under nitrogen starvation, mating was observed in both DL2 and DA2 transformed with the wild-type Zygo STE12. The expression of Zygo STE6 in Z. rouxii DL2 transformed with wild-type Zygo STE12 under nitrogen-starvation conditions was confirmed by lacZ reporter assay. Collectively, these results revealed that, under nitrogen-starvation conditions, Zygosaccharomyces sp. can mate and mating-type-specific genes are expressed. Furthermore, Zygo Ste12 is essential for both mating and the expression of mating-type-specific genes in Zygosaccharomyces sp"
Keywords:		"Amino Acid Sequence DNA, Fungal/genetics Fungal Proteins/*genetics Gene Expression *Gene Expression Regulation, Fungal Mating Factor/*genetics Nitrogen/metabolism Saccharomycetales/genetics Zygosaccharomyces/classification/*genetics/metabolism Zygosacchar;"
Notes:		"MedlineOgata, Tomoo Kuroki, Katsuaki eng England 2021/04/04 Yeast. 2021 Aug; 38(8):471-479. doi: 10.1002/yea.3561. Epub 2021 Apr 9"