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FEBS Lett

Title:		Stabilization of mRNA in an Escherichia coli cell-free translation system
Author(s):		Hirao I; Yoshizawa S; Miura K;
Address:		"Department of Industrial Chemistry, Faculty of Engineering, University of Tokyo, Japan"
Journal Title:		FEBS Lett
Year:		1993
Volume:		321
Issue:		2-Mar
Page Number:		169 - 172
DOI:		10.1016/0014-5793(93)80101-y
ISSN/ISBN:		0014-5793 (Print) 0014-5793 (Linking)
Abstract:		It became clear that mRNA can be stabilized in a cell-free translation system of Escherichia coli by hybridization with a small DNA fragment at its 3' terminus. The stability increased when a small DNA fragment containing a stable hairpin structure with a GAAA loop was used. The enhancement of stabilization was brought about because the hairpin structure is resistant towards the nucleases contained in the translation system. The hairpin structure is effective by stabilizing the added DNA fragment itself towards the nucleases
Keywords:		"Amino Acid Sequence Base Sequence Cell-Free System DNA, Bacterial/genetics Drug Stability Enterococcus faecalis/genetics Escherichia coli/genetics/*metabolism Macromolecular Substances Molecular Sequence Data Nucleic Acid Conformation Oligopeptides/biosyn;"
Notes:		"MedlineHirao, I Yoshizawa, S Miura, K eng Research Support, Non-U.S. Gov't England 1993/04/26 FEBS Lett. 1993 Apr 26; 321(2-3):169-72. doi: 10.1016/0014-5793(93)80101-y"