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Nucleic Acids Symp Ser

Title:		Stabilization of mRNA in the cell-free translation system from Escherichia coli
Author(s):		Hirao I; Yoshizawa S; Miura K;
Address:		"Department of Industrial Chemistry, Faculty of Engineering, University of Tokyo, Japan"
Journal Title:		Nucleic Acids Symp Ser
Year:		1990
Volume:
Issue:		22
Page Number:		75 - 76
DOI:
ISSN/ISBN:		0261-3166 (Print) 0261-3166 (Linking)
Abstract:		"In the RNA directed cell-free protein synthesizing system from E. coli, there is a problem of contaminating 3'-exonucleases which attack the mRNAs. Thus, we tried the following two methods to stabilize mRNAs against nucleases: (A) To use mRNAs having hairpin structures at their termini and (B) To hybridize mRNAs with small DNA fragments to the 3'-termini of mRNAs. It was found that degradation of a mRNA was inhibited by the method B rather than the method A in the translation system"
Keywords:		"Amino Acid Sequence Base Sequence Cell-Free System DNA, Bacterial/metabolism Escherichia coli/*genetics Exonucleases/metabolism Molecular Sequence Data Nucleic Acid Conformation Nucleic Acid Hybridization Oligopeptides/genetics Pheromones/genetics *Protei;"
Notes:		"MedlineHirao, I Yoshizawa, S Miura, K eng England 1990/01/01 Nucleic Acids Symp Ser. 1990; (22):75-6"