| Title: | Developmental and temporal changes in petunia petal transcriptome reveal scent-repressing plant-specific RING-kinase-WD40 protein |
| Author(s): | Shor E; Skaliter O; Sharon E; Kitsberg Y; Bednarczyk D; Kerzner S; Vainstein D; Tabach Y; Vainstein A; |
| Address: | "The Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, The Hebrew University of Jerusalem, Rehovot, Israel. The Institute for Medical Research, Israel-Canada, Hadassah Medical School, The Hebrew University of Jerusalem, Jerusalem, Israel. School of Computer Science, Tel Aviv University, Tel Aviv, Israel" |
| DOI: | 10.3389/fpls.2023.1180899 |
| ISSN/ISBN: | 1664-462X (Print) 1664-462X (Electronic) 1664-462X (Linking) |
| Abstract: | "In moth-pollinated petunias, production of floral volatiles initiates when the flower opens and occurs rhythmically during the day, for optimal flower-pollinator interaction. To characterize the developmental transcriptomic response to time of day, we generated RNA-Seq databases for corollas of floral buds and mature flowers in the morning and in the evening. Around 70% of transcripts accumulating in petals demonstrated significant changes in expression levels in response to the flowers' transition from a 4.5-cm bud to a flower 1 day postanthesis (1DPA). Overall, 44% of the petal transcripts were differentially expressed in the morning vs. evening. Morning/evening changes were affected by flower developmental stage, with a 2.5-fold larger transcriptomic response to daytime in 1DPA flowers compared to buds. Analyzed genes known to encode enzymes in volatile organic compound biosynthesis were upregulated in 1DPA flowers vs. buds-in parallel with the activation of scent production. Based on analysis of global changes in the petal transcriptome, PhWD2 was identified as a putative scent-related factor. PhWD2 is a protein that is uniquely present in plants and has a three-domain structure: RING-kinase-WD40. Suppression of PhWD2 (termed UPPER - Unique Plant PhEnylpropanoid Regulator) resulted in a significant increase in the levels of volatiles emitted from and accumulated in internal pools, suggesting that it is a negative regulator of petunia floral scent production" |
| Keywords: | daytime flower development petunia scent regulation transcriptome; |
| Notes: | "PubMed-not-MEDLINEShor, Ekaterina Skaliter, Oded Sharon, Elad Kitsberg, Yaarit Bednarczyk, Dominika Kerzner, Shane Vainstein, Danny Tabach, Yuval Vainstein, Alexander eng Switzerland 2023/06/26 Front Plant Sci. 2023 Jun 8; 14:1180899. doi: 10.3389/fpls.2023.1180899. eCollection 2023" |
