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J Biomol NMR

Title:		"Temperature-dependent spectral density analysis applied to monitoring backbone dynamics of major urinary protein-I complexed with the pheromone 2- sec-butyl-4,5-dihydrothiazole"
Author(s):		Krizova H; Zidek L; Stone MJ; Novotny MV; Sklenar V;
Address:		"National Centre for Biomolecular Research, Faculty of Science, Masaryk University, Kotlav rska 2, 61137 Brno, Czech Republic"
Journal Title:		J Biomol NMR
Year:		2004
Volume:		28
Issue:		4
Page Number:		369 - 384
DOI:		10.1023/B:JNMR.0000015404.61574.65
ISSN/ISBN:		0925-2738 (Print) 0925-2738 (Linking)
Abstract:		"Backbone dynamics of mouse major urinary protein I (MUP-I) was studied by (15)N NMR relaxation. Data were collected at multiple temperatures for a complex of MUP-I with its natural pheromonal ligand, 2- sec -4,5-dihydrothiazole, and for the free protein. The measured relaxation rates were analyzed using the reduced spectral density mapping. Graphical analysis of the spectral density values provided an unbiased qualitative picture of the internal motions. Varying temperature greatly increased the range of analyzed spectral density values and therefore improved reliability of the analysis. Quantitative parameters describing the dynamics on picosecond to nanosecond time scale were obtained using a novel method of simultaneous data fitting at multiple temperatures. Both methods showed that the backbone flexibility on the fast time scale is slightly increased upon pheromone binding, in accordance with the previously reported results. Zero-frequency spectral density values revealed conformational changes on the microsecond to millisecond time scale. Measurements at different temperatures allowed to monitor temperature dependence of the motional parameters"
Keywords:		"Animals Magnetic Resonance Spectroscopy Mice Models, Molecular Motion Protein Structure, Tertiary Proteins/*chemistry *Temperature Thiazoles/*chemistry;"
Notes:		"MedlineKrizova, Hana Zidek, Lukas Stone, Martin J Novotny, Milos V Sklenar, Vladimir eng DC 02418/DC/NIDCD NIH HHS/ Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S. Netherlands 2004/02/12 J Biomol NMR. 2004 Apr; 28(4):369-84. doi: 10.1023/B:JNMR.0000015404.61574.65"