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Curr Microbiol

Title:		Transfer of tetracycline resistance genes with aggregation substance in food-borne Enterococcus faecalis
Author(s):		Choi JM; Woo GJ;
Address:		"Laboratory of Food Safety and Evaluation, Department of Food Bioscience and Technology, Korea University, Anam-dong 5-ga, Seongbuk-Gu, Seoul, 136-713, Korea"
Journal Title:		Curr Microbiol
Year:		2015
Volume:		20141207
Issue:		4
Page Number:		476 - 484
DOI:		10.1007/s00284-014-0742-1
ISSN/ISBN:		1432-0991 (Electronic) 0343-8651 (Print) 0343-8651 (Linking)
Abstract:		"Enterococcus faecalis has the ability to conjugate with the aid of aggregation substance (AS) and inducible sex pheromones to exchange genetic elements in food matrix. To evaluate the food safety condition and the transferable factor, 250 tetracycline-resistant food-borne E. faecalis were collected in Korea. Among the isolates, a majority of tetracycline-resistant isolates (49.6 %) harbored both the tet(M) and tet(L) genes together, followed by tet(M) (19.6 %), and tet(L) (6.8 %) alone. Also, we found the combination of tet(L)/tet(M)/tet(O) or tet(M)/tet(O). We identified two tet(S) genes including the isolate carrying tet(M) + tet(S) genes. Additionally, most E. faecalis were positive for cpd and ccf (both 96.8 %) followed by cob (57.2 %). Through mating experiments, we confirmed E. faecalis possessing the Int-Tn gene and/or any AS gene successfully transferred tet genes to JH2-2 E. faecalis, whereas neither E. faecalis carrying AS genes nor the Int-Tn gene showed the conjugation. Pulsed-field gel electrophoresis results supported a distinct pattern, implying transfer of genetic information. Our study revealed a high occurrence of tetracycline resistance genes in E. faecalis from various foods. The widespread dissemination of tetracycline resistance genes would be promoted to transfer tetracycline resistance genes by pheromone-mediated conjugation systems"
Keywords:		"*Bacterial Adhesion Bacterial Proteins/genetics/*metabolism *Conjugation, Genetic Enterococcus faecalis/*drug effects/*genetics/physiology *Gene Transfer, Horizontal Korea *Tetracycline Resistance;"
Notes:		"MedlineChoi, Jong-Mi Woo, Gun-Jo eng Research Support, Non-U.S. Gov't 2014/12/10 Curr Microbiol. 2015 Apr; 70(4):476-84. doi: 10.1007/s00284-014-0742-1. Epub 2014 Dec 7"