| Title: | "Green Chemistry Production of Codlemone, the Sex Pheromone of the Codling Moth (Cydia pomonella), by Metabolic Engineering of the Oilseed Crop Camelina (Camelina sativa)" |
| Author(s): | Xia YH; Wang HL; Ding BJ; Svensson GP; Jarl-Sunesson C; Cahoon EB; Hofvander P; Lofstedt C; |
| Address: | "Department of Biology, Lund University, Solvegatan 37, 22362, Lund, Sweden. Division of Systems and Synthetic Biology, Department of Biology and Biological Engineering, Chalmers University of Technology, 41296, Gothenburg, Sweden. Department of Biochemistry and Center for Plant Science Innovation, University of Nebraska-Lincoln, Lincoln, NE, 68588, USA. Department of Plant Breeding, Swedish University of Agricultural Sciences, P.O. Box 101, 23053, Alnarp, Sweden. Department of Biology, Lund University, Solvegatan 37, 22362, Lund, Sweden. christer.lofstedt@biol.lu.se" |
| DOI: | 10.1007/s10886-021-01316-4 |
| ISSN/ISBN: | 1573-1561 (Electronic) 0098-0331 (Print) 0098-0331 (Linking) |
| Abstract: | "Synthetic pheromones have been used for pest control over several decades. The conventional synthesis of di-unsaturated pheromone compounds is usually complex and costly. Camelina (Camelina sativa) has emerged as an ideal, non-food biotech oilseed platform for production of oils with modified fatty acid compositions. We used Camelina as a plant factory to produce mono- and di-unsaturated C(12) chain length moth sex pheromone precursors, (E)-9-dodecenoic acid and (E,E)-8,10-dodecadienoic acid, by introducing a fatty acyl-ACP thioesterase FatB gene UcTE from California bay laurel (Umbellularia californica) and a bifunctional ?Os9 desaturase gene Cpo_CPRQ from the codling moth, Cydia pomonella. Different transgene combinations were investigated for increasing pheromone precursor yield. The most productive Camelina line was engineered with a vector that contained one copy of UcTE and the viral suppressor protein encoding P19 transgenes and three copies of Cpo_CPRQ transgene. The T(2) generation of this line produced 9.4% of (E)-9-dodecenoic acid and 5.5% of (E,E)-8,10-dodecadienoic acid of the total fatty acids, and seeds were selected to advance top-performing lines to homozygosity. In the T(4) generation, production levels of (E)-9-dodecenoic acid and (E,E)-8,10-dodecadienoic acid remained stable. The diene acid together with other seed fatty acids were converted into corresponding alcohols, and the bioactivity of the plant-derived codlemone was confirmed by GC-EAD and a flight tunnel assay. Trapping in orchards and home gardens confirmed significant and specific attraction of C. pomonella males to the plant-derived codlemone" |
| Keywords: | Animals Brassicaceae/*chemistry Dodecanol/*analogs & derivatives/chemistry/metabolism *Metabolic Engineering Moths/*drug effects Sex Attractants/chemistry/*pharmacology Acyl-ACP thioesterase Agrobacterium-based floral-dip transformation Bioassay Conjugate; |
| Notes: | "MedlineXia, Yi-Han Wang, Hong-Lei Ding, Bao-Jian Svensson, Glenn P Jarl-Sunesson, Carin Cahoon, Edgar B Hofvander, Per Lofstedt, Christer eng RBP 14-0037/stiftelsen for strategisk forskning/ No. 760798/horizon 2020 framework programme/ CTS 14:307/carl tryggers stiftelse for vetenskaplig forskning/ CTS KF17:15/carl tryggers stiftelse for vetenskaplig forskning/ 2010-857/svenska forskningsradet formas/ 2015-1336/svenska forskningsradet formas/ NEB-30-131/nebraska agricultural experiment station-usda hatch act/ 2021/11/12 J Chem Ecol. 2021 Dec; 47(12):950-967. doi: 10.1007/s10886-021-01316-4. Epub 2021 Nov 11" |
