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J Chem Ecol

Title:		Multi-Functional Desaturases in Two Spodoptera Moths with ?Os11 and ?Os12 Desaturation Activities
Author(s):		Xia YH; Zhang YN; Ding BJ; Wang HL; Lofstedt C;
Address:		"Department of Biology, Lund University, Solvegatan 37, SE-223 62, Lund, Sweden. yihan.xia@biol.lu.se. College of Life Sciences, Huaibei Normal University, Dongshan Road 100, Huaibei, CN-235000, China. Department of Biology, Lund University, Solvegatan 37, SE-223 62, Lund, Sweden"
Journal Title:		J Chem Ecol
Year:		2019
Volume:		20190402
Issue:		4
Page Number:		378 - 387
DOI:		10.1007/s10886-019-01067-3
ISSN/ISBN:		1573-1561 (Electronic) 0098-0331 (Print) 0098-0331 (Linking)
Abstract:		"The beet armyworm, Spodoptera exigua, uses (Z,E)-9,12-tetradecadienyl acetate as the major component of its sex pheromone. Previous isotope-labeling experiments demonstrated an unusual ?Os12 desaturase activity involved in the biosynthesis of this compound; however, the putative ?Os12 desaturase gene has not been reported to date. In the present study, we confirmed this ?Os12 desaturation pathway by in vivo labeling experiments, and characterized candidate desaturase genes in a yeast heterologous expression system. We demonstrated that a pheromone gland-specific desaturase, SexiDes5, uses palmitic acid and the subsequently chain-shortened product (Z)-9-tetradecenoic acid as substrates to produce (Z)-11-hexadecenoic and (Z,E)-9,12-tetradecadienoic acids, respectively. In addition, the homologous desaturase SlitDes5 from the congeneric Spodoptera litura had analogous functions"
Keywords:		"Animals Cloning, Molecular Fatty Acid Desaturases/genetics/*metabolism Female Gas Chromatography-Mass Spectrometry Insect Proteins/genetics/*metabolism Male Saccharomyces cerevisiae/genetics Sex Attractants/metabolism Species Specificity Spodoptera/classi;"
Notes:		"MedlineXia, Yi-Han Zhang, Ya-Nan Ding, Bao-Jian Wang, Hong-Lei Lofstedt, Christer eng none/Svenska Forskningsradet Formas/ 31501647/National Natural Science Foundation of China/ KJ2017A387 and KJ2017B019/Natural Science Foundation of Anhui Province/ 2019/04/03 J Chem Ecol. 2019 Apr; 45(4):378-387. doi: 10.1007/s10886-019-01067-3. Epub 2019 Apr 2"