| Title: | "Degradation of sex pheromone and plant volatile components by an antennal glutathione S-transferase in the oriental fruit moth,Grapholita molesta Busck (Lepidoptera: Tortricidae)" |
| Author(s): | Li GW; Chen XL; Xu XL; Wu JX; |
| Address: | "Shaanxi Province Key Laboratory of Jujube, College of Life Science, Yan'an University, Yan'an, China. Key Laboratory of Plant Protection Resources and Pest Management (Northwest A&F University), Ministry of Education, Yangling, China. Key Laboratory of Applied Entomology, College of Plant Protection, Northwest A&F University, Yangling, China" |
| Journal Title: | Arch Insect Biochem Physiol |
| ISSN/ISBN: | 1520-6327 (Electronic) 0739-4462 (Linking) |
| Abstract: | "Insect antennae have a primary function of perceiving and discerning odorant molecules including sex pheromones and host plant volatiles. The assumption that genes highly expressed in the antennae may have an olfactory-related role associated with signal transduction. Here, one delta subfamily glutathione S-transferase (GST) gene (GmolGSTD1) was obtained from an antennal transcriptome of Grapholita molesta. Quantitative real-time polymerase chain reaction results revealed that GmolGSTD1 was mainly expressed in antennae and the expression levels were significantly higher in female antennae than in male antennae. The recombinant GmolGSTD1 (rGmolGSTD1) showed glutathione-conjugating activity toward 1-chloro-2,4-dinitrobenzene (CDNB) as substrates. The pH range for optimal rGmolGSTD1 enzyme activity was 6.0-6.5, and rGmolGSTD1 enzyme activity had maximal peaks at 35-40 degrees C. Spectrophotometric analysis indicated that insecticides had weak inhibitory effects on the activity of rGmolGSTD1 with the inhibitory rates of 28.82% for chlorpyrifos, 22.27% for lambda-cyhalothrin, 18.07% for bifenthrin, 20.42% for acetamiprid, 17.57% for thiamethoxam, 25.67% for metaflumizone, 27.43% for abamectin, and 7.24% for chlorbenzuron. rGmolGSTD1 exhibited high degradation activity to the sex pheromone component (Z)-8-dodecenyl alcohol and the host plant volatile butyl hexanoate with the degradation efficiency of 75.01% and 48.54%, respectively. We speculate that GmolGSTD1 works in inactivating odorant molecules and maintaining sensitivity to olfactory communication of G. molesta" |
| Keywords: | "Animals Arthropod Antennae/*enzymology Female Glutathione Transferase/*metabolism Insect Proteins/metabolism Male Moths/*physiology Receptors, Odorant/physiology Sex Attractants/*metabolism Grapholita molesta glutathione S-transferase olfactory signal dea;" |
| Notes: | "MedlineLi, Guang-Wei Chen, Xiu-Lin Xu, Xiang-Li Wu, Jun-Xiang eng YDBK2017-37/Ph.D. Research Startup Foundation of Yan'an University/ 31860506/National Natural Science Foundation of China/ 31272043/National Natural Science Foundation of China/ 2017cxy04/Project of Industry-University-Research Collaboration of Yan' an University in 2017/ 2018/11/06 Arch Insect Biochem Physiol. 2018 Dec; 99(4):e21512. doi: 10.1002/arch.21512. Epub 2018 Nov 2" |
