| Title: | "CLONING, EXPRESSION, AND FUNCTIONAL ANALYSIS OF THREE ODORANT-BINDING PROTEINS OF THE ORIENTAL FRUIT MOTH, Grapholita molesta (BUSCK) (LEPIDOPTERA: TORTRICIDAE)" |
| Author(s): | Li GW; Zhang Y; Li YP; Wu JX; Xu XL; |
| Address: | "Key Laboratory of Plant Protection Resources and Pest Management (Northwest A&F University), Ministry of Education, Yangling, China. Key Laboratory of Applied Entomology, Northwest A&F University, Yangling, China" |
| Journal Title: | Arch Insect Biochem Physiol |
| ISSN/ISBN: | 1520-6327 (Electronic) 0739-4462 (Linking) |
| Abstract: | "Odorant-binding proteins (OBPs) act in insect olfactory processes. OBPs are expressed in the olfactory organs and serve in binding and transport of hydrophobic odorants through the sensillum lymph to olfactory receptor neurons within the antennal sensilla. In this study, three OBP genes were cloned from the antennal transcriptome database of Grapholita molesta via reverse-transcription PCR. Recombinant GmolOBPs (rGmolOBPs) were expressed in a prokaryotic expression system and enriched via Ni ion affinity chromatography. The binding properties of the three rGmolOBPs to four sex pheromones and 30 host-plant volatiles were investigated in fluorescence ligand-binding assays. The results demonstrated that rGmolOBP8, rGmolOBP11, and rGmolOBP15 exhibited high binding affinities with the major sex pheromone components (E)-8-dodecenyl acetate, (Z)-8-dodecenyl alcohol, and dodecanol. The volatiles emitted from peach and pear, decanal, butyl hexanoate, and alpha-ocimene, also showed binding affinities to rGmolOBP8 and rGmolOBP11. Hexanal, heptanal, and alpha-pinene showed strong binding affinities to rGmolOBP15. Results of the electrophysiological recording experiments and previous behavior bioassays indicated that adult insects had strong electroantennogram and behavioral responses toward butyl hexanoate, hexanal, and heptanal. We infer that the GmolOBP8 and GmolOBP11 have dual functions in perception and recognition of host-plant volatiles and sex pheromones, while GmolOBP15 was mainly involved in plant volatile odorants' perception" |
| Keywords: | "Amino Acid Sequence Animals Arthropod Antennae/*metabolism Base Sequence Chromatography, Affinity Electrophysiological Phenomena Female Insect Proteins/chemistry/genetics/metabolism Male Molecular Sequence Data Moths/genetics/*metabolism Receptors, Odoran;" |
| Notes: | "MedlineLi, Guang-Wei Zhang, Yan Li, Yi-Ping Wu, Jun-Xiang Xu, Xiang-Li eng Research Support, Non-U.S. Gov't 2015/11/27 Arch Insect Biochem Physiol. 2016 Feb; 91(2):67-87. doi: 10.1002/arch.21309. Epub 2015 Nov 26" |
