| Title: | "Volatile organic compounds produced by a soil-isolate, Bacillus subtilis FA26 induce adverse ultra-structural changes to the cells of Clavibacter michiganensis ssp. sepedonicus, the causal agent of bacterial ring rot of potato" |
| Author(s): | Rajer FU; Wu H; Xie Y; Xie S; Raza W; Tahir HAS; Gao X; |
| Address: | "Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing 210095, PR China" |
| ISSN/ISBN: | 1465-2080 (Electronic) 1350-0872 (Linking) |
| Abstract: | "Rhizobacterial volatile organic compounds (VOCs) play an important role in the suppression of soil-borne phytopathogens. In this study, the VOCs produced by a soil-isolate, Bacillus subtilis FA26, were evaluated in vitro for their antibacterial activity against Clavibacter michiganensis ssp. sepedonicus (Cms), the causal agent of bacterial ring rot of potato. The VOCs emitted by FA26 inhibited the growth of Cms significantly compared with the control. Scanning and transmission electron microscopy analyses revealed distorted colony morphology and a wide range of abnormalities in Cms cells exposed to the VOCs of FA26. Varying the inoculation strategy and inoculum size showed that the production and activity of the antibacterial VOCs of FA26 were dependent on the culture conditions. Headspace solid-phase microextraction/gas chromatography-mass spectrometry analyses revealed that FA26 produced 11 VOCs. Four VOCs (benzaldehyde, nonanal, benzothiazole and acetophenone) were associated with the antibacterial activity against Cms. The results suggested that the VOCs produced by FA26 could control the causal agent of bacterial ring rot of potato. This information will increase our understanding of the microbial interactions mediated by VOCs in nature and aid the development of safer strategies for controlling plant disease" |
| Keywords: | Acetophenones/metabolism/pharmacology Aldehydes/metabolism/pharmacology Anti-Bacterial Agents/biosynthesis/*pharmacology Bacillus subtilis/*metabolism Benzaldehydes/metabolism/pharmacology Benzothiazoles/metabolism/pharmacology Gas Chromatography-Mass Spe; |
| Notes: | "MedlineRajer, Faheem Uddin Wu, Huijun Xie, Yongli Xie, Shanshan Raza, Waseem Tahir, Hafiz Abdul Samad Gao, Xuewen eng Research Support, Non-U.S. Gov't England 2017/04/19 Microbiology (Reading). 2017 Apr; 163(4):523-530. doi: 10.1099/mic.0.000451. Epub 2017 Apr 18" |
