| Title: | Antifungal susceptibility and virulence profile of candida isolates from abnormal vaginal discharge of women from southern India |
| Author(s): | Ganeshkumar A; Nagarajan P; Mahalingam P; Balasubramanian S; Archunan PA; Govindaraju A; Rajaram R; |
| Address: | "DNA Barcoding and Marine Genomics Laboratory, Department of Marine Sciences, Bharathidasan University, Tiruchirapalli, India; Department of Microbiology, Trichy SRM Medical College Hospital and Research Centre, Tiruchirapalli, India. Department of Microbiology, Trichy SRM Medical College Hospital and Research Centre, Tiruchirapalli, India. Department of Microbiology, Trichy SRM Medical College Hospital and Research Centre, Tiruchirapalli, India; Department of Biotechnology, Nehru Memorial College, Tiruchirapalli, India. Department of Obstetrics and Gynaecology, Trichy SRM Medical College Hospital and Research Centre, Tiruchirapalli, India. Department of Obstetrics and Gynaecology, R.N.T Medical College, Udaipur, India. Centre for Pheromone Technology, Department of Animal Sciences, Bharathidasan University, Tiruchirapalli, India. DNA Barcoding and Marine Genomics Laboratory, Department of Marine Sciences, Bharathidasan University, Tiruchirapalli, India. Electronic address: drrajaram69@rediffmail.com" |
| Journal Title: | Eur J Obstet Gynecol Reprod Biol |
| DOI: | 10.1016/j.ejogrb.2020.09.021 |
| ISSN/ISBN: | 1872-7654 (Electronic) 0301-2115 (Linking) |
| Abstract: | "OBJECTIVE: Vaginal candidiasis is the most common opportunistic fungal infection, largely associated with a woman's psychological and economic status. Recently, the rate of disease progression has increased extensively; however, region-specific studies are very limited. This study aimed to understand variation in hydrolytic activities and antifungal susceptibility of Candida isolates from vaginal discharge. STUDY DESIGN: The present study was aimed to understand the variation of hydrolytic activities and antifungal susceptibility of Candida isolates from vaginal discharge. RESULTS: In total, 34 different Candida isolates were collected: C. albicans (n = 17; 50 %), C. glabrata (n = 6; 17.64 %), C. tropicalis (n = 4; 11.76 %) and C. parapsilosis (n = 7; 20.58 %). All isolates were primarily identified and confirmed by basic microbiological methods followed by the VITEK-2 system. Antifungal susceptibility of the isolates were evaluated using yeast antifungal susceptibility testing cards. The isolates of C. albicans, C. glabrata, C. tropicalis and C. parapsilosis were 100 % susceptible to amphotericin B. The non-albicans isolates presented 100 % of proteolytic and lipolytic activity compared with C. albicans. Eight (47.06 %) C. albicans isolates showed positive esterase activity (Pz<1), whereas nine (52.94 %) were negative to esterase (Pz = 1). Of the 34 Candida isolates, 28 (82.35 %) were found to be moderate-to-strong biofilm producers: 14 C. albicans, three C. glabrata, six C. parapsilosis and five C. tropicalis. CONCLUSION: This study clarified the antifungal susceptibility and virulence behaviour of Candida isolates; this will be of use in the selection of antifungal agents for Candida prophylaxis" |
| Keywords: | "*Antifungal Agents/pharmacology Candida Drug Resistance, Fungal Female Humans India/epidemiology Microbial Sensitivity Tests *Vaginal Discharge Virulence Antifungal susceptibility Candida isolates Hydrolytic activity Vaginal discharge;" |
| Notes: | "MedlineGaneshkumar, Arumugam Nagarajan, Prabhusaran Mahalingam, Pramila Balasubramanian, Saranya Archunan, Priya Aarthy Govindaraju, Archunan Rajaram, Rajendran eng Ireland 2020/09/25 Eur J Obstet Gynecol Reprod Biol. 2020 Nov; 254:153-158. doi: 10.1016/j.ejogrb.2020.09.021. Epub 2020 Sep 16" |
