| Title: | Adverse effects of electronic cigarettes on the disease-naive oral microbiome |
| Author(s): | Ganesan SM; Dabdoub SM; Nagaraja HN; Scott ML; Pamulapati S; Berman ML; Shields PG; Wewers ME; Kumar PS; |
| Address: | "Division of Periodontology, The Ohio State University, Columbus, OH, USA. College of Public Health, The Ohio State University, Columbus, OH, USA. Moritz College of Law, The Ohio State University, Columbus, OH, USA. Wexner Medical Center, The Ohio State University, Columbus, OH, USA. James Comprehensive Cancer Center, The Ohio State University, Columbus, OH, USA" |
| ISSN/ISBN: | 2375-2548 (Electronic) 2375-2548 (Linking) |
| Abstract: | "Six percent of Americans, including 3 million high schoolers, use e-cigarettes, which contain potentially toxic substances, volatile organic compounds, and metals. We present the first human study on the effects of e-cigarette exposure in the oral cavity. By interrogating both immunoinflammatory responses and microbial functional dynamics, we discovered pathogen overrepresentation, higher virulence signatures, and a brisk proinflammatory signal in clinically healthy e-cigarette users, equivalent to patients with severe periodontitis. Using RNA sequencing and confocal and electron microscopy to validate these findings, we demonstrate that the carbon-rich glycol/glycerol vehicle is an important catalyst in transforming biofilm architecture within 24 hours of exposure. Last, a machine-learning classifier trained on the metagenomic signatures of e-cigarettes identified as e-cigarette users both those individuals who used e-cigarettes to quit smoking, and those who use both e-cigarettes and cigarettes. The present study questions the safety of e-cigarettes and the harm reduction narrative promoted by advertising campaigns" |
| Keywords: | *Electronic Nicotine Delivery Systems Humans *Microbiota Smoking *Tobacco Products/adverse effects United States *Volatile Organic Compounds; |
| Notes: | "MedlineGanesan, Sukirth M Dabdoub, Shareef M Nagaraja, Haikady N Scott, Michelle L Pamulapati, Surya Berman, Micah L Shields, Peter G Wewers, Mary Ellen Kumar, Purnima S eng R01 DE027857/DE/NIDCR NIH HHS/ Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't 2020/06/11 Sci Adv. 2020 May 27; 6(22):eaaz0108. doi: 10.1126/sciadv.aaz0108. eCollection 2020 May" |
