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World J Microbiol Biotechnol

Title:		Antifungal activity of volatile compounds-producing Pseudomonas P2 strain against Rhizoctonia solani
Author(s):		Elkahoui S; Djebali N; Yaich N; Azaiez S; Hammami M; Essid R; Limam F;
Address:		"Laboratoire des Substances Bioactives, Centre de Biotechnologie de Borj-Cedria, BP-901, 2050, Hammam-Lif, Tunisia"
Journal Title:		World J Microbiol Biotechnol
Year:		2015
Volume:		20141111
Issue:		1
Page Number:		175 - 185
DOI:		10.1007/s11274-014-1772-3
ISSN/ISBN:		1573-0972 (Electronic) 0959-3993 (Linking)
Abstract:		"Several volatile organic compounds (VOCs) producing endophyte bacteria were isolated from the leaves of olive trees and tested for their antifungal activity against several pathogenic fungi. An antagonistic strain called P2 showed 97 % of homology with Pseudomonas sp. strains on the basis of its 16S rDNA sequence and biochemical properties. P2 strain drastically inhibited the growth of Rhizoctonia solani mycelia (86 %) at 5 day-post-confrontation (dpc) and strongly reduced fungi infection on potato slices at 10(7) bacteria ml(-1) for 3 and 7 dpc. P2 strain was also positive for protease activity as well as siderophore production. Light microscopy analysis showed that treatment of R. solani mycelia with P2 strain induced thickening of the cell-wall, vesiculation of protoplasm and blockage of fungal hyphae branching. VOCs analysis using GC-MS allowed the detection of two major products with m/z of 93.9910 and 125.9630 corresponding to dimethyl disulfide and dimethyl trisulfide respectively. VOCs-producing P2 strain could be a promising agent in the protection of tuber crops against fungal diseases"
Keywords:		"Antifungal Agents/*metabolism Bacterial Typing Techniques Base Composition Cluster Analysis DNA, Bacterial/chemistry/genetics DNA, Ribosomal/chemistry/genetics Microscopy Molecular Sequence Data Phylogeny Pseudomonas/classification/genetics/isolation & pu;"
Notes:		"MedlineElkahoui, Salem Djebali, Naceur Yaich, Najeh Azaiez, Sana Hammami, Majdi Essid, Rym Limam, Ferid eng Research Support, Non-U.S. Gov't Germany 2014/11/12 World J Microbiol Biotechnol. 2015 Jan; 31(1):175-85. doi: 10.1007/s11274-014-1772-3. Epub 2014 Nov 11"