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Biosci Biotechnol Biochem

Title:		Purification and structural analysis of volatile sesquiterpenes produced by Escherichia coli carrying unidentified terpene synthase genes from edible plants of the family Araliaceae
Author(s):		Shindo K; Hattan JI; Kato M; Sato M; Ito T; Shibuya Y; Watanabe A; Sugiyama M; Nakamura Y; Misawa N;
Address:		"a Department of Food and Nutrition , Japan Women's University , Tokyo , Japan. b Research Institute for Bioresources and Biotechnology , Ishikawa Prefectural University , Ishikawa , Japan"
Journal Title:		Biosci Biotechnol Biochem
Year:		2018
Volume:		20171122
Issue:		6
Page Number:		978 - 985
DOI:		10.1080/09168451.2017.1386085
ISSN/ISBN:		1347-6947 (Electronic) 0916-8451 (Linking)
Abstract:		"A simple method to purify volatile sesquiterpenes from recombinant Escherichia coli was developed using the cells that carried known sesquiterpene synthase (Tps) genes ZzZss2 (ZSS2) and ZoTps1. This method was applied for the purification and structural analyses of volatile sesquiterpenes produced by E. coli cells that carried unidentified Tps genes, which were isolated from the Aralia-genus edible plants belonging to the family Araliaceae. Recombinant cells carrying each Tps gene were cultured in the two-layer medium (n-octane/TB medium), and volatile sesquiterpenes trapped in n-octane were purified through two-phase partition, silica gel column chromatography, and reversed-phase preparative high-performance liquid chromatography, if necessary. Further, their structures were confirmed by nuclear magnetic resonance, [alpha](D), and gas chromatography-mass spectrometry analyses. Herein, the products of E. coli cells that carried two Tps gene (named AcTps1 and AcTps2) in Araria cordata 'Udo' and a Tps gene (named AeTps1) in Aralia elata 'Taranoki' were studied resulting in identifying functionalities of these cryptic Tps genes"
Keywords:		"Alkyl and Aryl Transferases/*genetics Araliaceae/*genetics Carbon-13 Magnetic Resonance Spectroscopy Chromatography, Liquid/methods Culture Media Escherichia coli/genetics/*metabolism Fermentation Gas Chromatography-Mass Spectrometry Molecular Structure P;"
Notes:		"MedlineShindo, Kazutoshi Hattan, Jun-Ichiro Kato, Mariko Sato, Miho Ito, Tomoko Shibuya, Yurika Watanabe, Arisa Sugiyama, Maki Nakamura, Yuri Misawa, Norihiko eng England 2017/11/23 Biosci Biotechnol Biochem. 2018 Jun; 82(6):978-985. doi: 10.1080/09168451.2017.1386085. Epub 2017 Nov 22"