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Mol Microbiol

Title:		Target evaluation of the non-coding csRNAs reveals a link of the two-component regulatory system CiaRH to competence control in Streptococcus pneumoniae R6
Author(s):		Schnorpfeil A; Kranz M; Kovacs M; Kirsch C; Gartmann J; Brunner I; Bittmann S; Bruckner R;
Address:		"Department of Microbiology, University of Kaiserslautern, D-67663, Kaiserslautern, Germany"
Journal Title:		Mol Microbiol
Year:		2013
Volume:		20130614
Issue:		2
Page Number:		334 - 349
DOI:		10.1111/mmi.12277
ISSN/ISBN:		1365-2958 (Electronic) 0950-382X (Linking)
Abstract:		"The two-component regulatory system CiaRH of Streptococcus pneumoniae controls 25 genes, five of which specify homologous small non-coding csRNAs (cia-dependent small RNAs). The csRNAs were predicted to act regulatory as base-pairing sRNAs, but their targets have not been identified. By csRNA gene inactivations we established that the major phenotypes associated with a hyperactive CiaRH system, enhanced beta-lactam resistance and prevention of genetic competence, are dependent on the csRNAs. Computational target predictions and evaluations by translational fusions identified six genes to be under csRNA control: spr0081, spr0371, spr0159, spr0551, spr1097 and spr2043(comC). Measuring the effect of single csRNAs on three targets indicated that they acted additively. One of the targets, comC(spr2043), encoding the precursor of the competence stimulating pheromone CSP, constitutes a link of CiaRH to competence control. Partially disrupting predicted csRNA-comC complementarity led to strongly diminished repression by the csRNAs and to transformability in a strain with a hyperactive CiaRH. Thus, a hyperactive CiaRH system prevents competence development by csRNA-dependent post-transcriptional repression of CSP production. The csRNAs are also involved in competence regulation in the wild-type strain R6, but their activity is only apparent in the absence of the protease gene htrA, another CiaRH regulon member"
Keywords:		"Bacterial Proteins/chemistry/*genetics/*metabolism *Gene Expression Regulation, Bacterial Gene Targeting Promoter Regions, Genetic Protein Kinases/chemistry/*genetics/metabolism RNA, Bacterial/chemistry/genetics RNA, Small Untranslated/chemistry/*genetics;"
Notes:		"MedlineSchnorpfeil, Anke Kranz, Miriam Kovacs, Marta Kirsch, Christian Gartmann, Julia Brunner, Ines Bittmann, Sabrina Bruckner, Reinhold eng Research Support, Non-U.S. Gov't England 2013/05/29 Mol Microbiol. 2013 Jul; 89(2):334-49. doi: 10.1111/mmi.12277. Epub 2013 Jun 14"