| Title: | Two pheromone precursor genes are transcriptionally expressed in the homothallic ascomycete Sordaria macrospora |
| Address: | "Lehrstuhl fur Allgemeine und Molekulare Botanik, Ruhr-Universitat Bochum, Germany. Stefanie.Poeggeler@ruhr-uni-bochum.de" |
| ISSN/ISBN: | 0172-8083 (Print) 0172-8083 (Linking) |
| Abstract: | "In order to analyze the involvement of pheromones in cell recognition and mating in a homothallic fungus, two putative pheromone precursor genes, named ppg1 and ppg2, were isolated from a genomic library of Sordaria macrospora. The ppg1 gene is predicted to encode a precursor pheromone that is processed by a Kex2-like protease to yield a pheromone that is structurally similar to the alpha-factor of the yeast Saccharomyces cerevisiae. The ppg2 gene encodes a 24-amino-acid polypeptide that contains a putative farnesylated and carboxy methylated C-terminal cysteine residue. The sequences of the predicted pheromones display strong structural similarity to those encoded by putative pheromones of heterothallic filamentous ascomycetes. Both genes are expressed during the life cycle of S. macrospora. This is the first description of pheromone precursor genes encoded by a homothallic fungus. Southern-hybridization experiments indicated that ppg1 and ppg2 homologues are also present in other homothallic ascomycetes" |
| Keywords: | "Amino Acid Sequence Base Sequence Blotting, Northern Blotting, Southern Fungal Proteins *Genes, Fungal Molecular Sequence Data Pheromones/*genetics/metabolism/physiology Polymerase Chain Reaction Protein Precursors/*genetics/metabolism RNA, Fungal/analysi;" |
| Notes: | "MedlinePoggeler, S eng Research Support, Non-U.S. Gov't 2000/07/25 Curr Genet. 2000 Jun; 37(6):403-11. doi: 10.1007/s002940000120" |
