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J Basic Microbiol

Title:		Virulence factors and bacteriocins in faecal enterococci of wild boars
Author(s):		Poeta P; Igrejas G; Costa D; Sargo R; Rodrigues J; Torres C;
Address:		"Universidade de Tras-os-Montes e Alto Douro, Departamento de Ciencias Veterinarias, Vila Real, Portugal"
Journal Title:		J Basic Microbiol
Year:		2008
Volume:		48
Issue:		5
Page Number:		385 - 392
DOI:		10.1002/jobm.200700327
ISSN/ISBN:		0233-111X (Print) 0233-111X (Linking)
Abstract:		"The production of antimicrobial, haemolytic and gelatinase activities was tested in 67 enterococci (39 E. faecium, 24 E. hirae, 2 E. faecalis, and 2 Enterococcus spp.), recovered from faecal samples of wild boars. In addition, the presence of genes encoding bacteriocin and virulence factors was also analysed by PCR and sequencing. Production of antimicrobial activity was checked in all enterococci against 9 indicator bacteria and it was detected in 11 E. faecium isolates (16.5%); eight and two of them harboured the genes encoding enterocin A + enterocin B and enterocin L50A/B, respectively. Sixty-seven per cent of our enterococci harboured different combinations of genes of the cyl operon, but none of them contained the complete cyl L(L)L(S)ABM operon, necessary for cytolysin expression. The presence of gel E gene, associated with the fsr ABC locus, was identified in 4 E. faecium and two E. faecalis isolates, exhibiting all of them gelatinase activity. beta -hemolytic activity was not found in our isolates. Both cpd and ace genes, encoding respectively the accessory colonisation factor and pheromone, were detected in two E. faecalis isolates, and the hyl gene, encoding hyalorunidase, in two E. faecium isolates, one of them gelatinase-positive. Genes encoding bacteriocins and virulence factors are widely disseminated among faecal enterococci of wild boars and more studies should be carried out to know the global distribution of these determinants in enterococci of different ecosystems"
Keywords:		"Animals Bacteriocins/*genetics/metabolism Enterococcus/*genetics/metabolism Feces/microbiology Gelatinases/genetics/metabolism Genes, Bacterial Genotype Hemolysin Proteins/genetics/metabolism Phenotype Portugal Sus scrofa/*microbiology Virulence Factors/*;"
Notes:		"MedlinePoeta, Patricia Igrejas, Gilberto Costa, Daniela Sargo, Roberto Rodrigues, Jorge Torres, Carmen eng Research Support, Non-U.S. Gov't Germany 2008/09/02 J Basic Microbiol. 2008 Oct; 48(5):385-92. doi: 10.1002/jobm.200700327"