| Title: | Sperm Metabolomics through Nuclear Magnetic Resonance Spectroscopy |
| Author(s): | Lombo M; Ruiz-Diaz S; Gutierrez-Adan A; Sanchez-Calabuig MJ; |
| Address: | "Department of Animal Reproduction, INIA, Av. Puerta de Hierro, 18, 28040 Madrid, Spain. Mistral Fertility Clinics S.L., Clinica Tambre, 28002 Madrid, Spain. Department of Animal Medicine and Surgery, Faculty of Veterinary Science, University Complutense of Madrid, 28040 Madrid, Spain" |
| ISSN/ISBN: | 2076-2615 (Print) 2076-2615 (Electronic) 2076-2615 (Linking) |
| Abstract: | "This report reviews current knowledge of sperm metabolomics analysis using proton nuclear magnetic resonance spectroscopy ((1) H-NMR) with particular emphasis on human and farm animals. First, we present the benefits of NMR over other techniques to identify sperm metabolites and then describe the specific methodology required for NMR sperm analysis, stressing the importance of analyzing metabolites extracted from both the hydrophilic and lipophilic phases. This is followed by a description of advances produced to date in the use of NMR to diagnose infertility in humans and to identify metabolic differences among the sperm of mammalian herbivore, carnivore, and omnivore species. This last application of NMR mainly seeks to explore the possible use of lipids to fuel sperm physiology, contrary to previous theories that glycolysis and oxidative phosphorylation (OXPHOS) are the only sources of sperm energy. This review describes the use of NMR to identify sperm and seminal plasma metabolites as possible indicators of semen quality, and to examine the metabolites needed to maintain sperm motility, induce their capacitation, and consequently, to predict animal fertility" |
| Keywords: | Animals;Nmr male fertility mammalian species sperm metabolites; |
| Notes: | "PubMed-not-MEDLINELombo, Marta Ruiz-Diaz, Sara Gutierrez-Adan, Alfonso Sanchez-Calabuig, Maria-Jesus eng RTI2018-093548-BI00/Spanish Ministry of Science and Innovation (MICINN)/ Review Switzerland 2021/07/03 Animals (Basel). 2021 Jun 3; 11(6):1669. doi: 10.3390/ani11061669" |
