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Genetics

Title:		"A dual, catalytic role for the fission yeast Ccr4-Not complex in gene silencing and heterochromatin spreading"
Author(s):		Challal D; Menant A; Goksal C; Leroy E; Al-Sady B; Rougemaille M;
Address:		"Universite Paris-Saclay, CEA, CNRS, Institute for Integrative Biology of the Cell (I2BC), Gif-sur-Yvette 91198, France. Department of Microbiology & Immunology, George Williams Hooper Foundation, University of California San Francisco, San Francisco, CA 94143, USA"
Journal Title:		Genetics
Year:		2023
Volume:		224
Issue:		4
Page Number:		-
DOI:		10.1093/genetics/iyad108
ISSN/ISBN:		1943-2631 (Electronic) 0016-6731 (Print) 0016-6731 (Linking)
Abstract:		"Heterochromatic gene silencing relies on combinatorial control by specific histone modifications, the occurrence of transcription, and/or RNA degradation. Once nucleated, heterochromatin propagates within defined chromosomal regions and is maintained throughout cell divisions to warrant proper genome expression and integrity. In the fission yeast Schizosaccharomyces pombe, the Ccr4-Not complex partakes in gene silencing, but its relative contribution to distinct heterochromatin domains and its role in nucleation versus spreading have remained elusive. Here, we unveil major functions for Ccr4-Not in silencing and heterochromatin spreading at the mating type locus and subtelomeres. Mutations of the catalytic subunits Caf1 or Mot2, involved in RNA deadenylation and protein ubiquitinylation, respectively, result in impaired propagation of H3K9me3 and massive accumulation of nucleation-distal heterochromatic transcripts. Both silencing and spreading defects are suppressed upon disruption of the heterochromatin antagonizing factor Epe1. Overall, our results position the Ccr4-Not complex as a critical, dual regulator of heterochromatic gene silencing and spreading"
Keywords:		*Schizosaccharomyces/genetics/metabolism Heterochromatin/genetics/metabolism *Schizosaccharomyces pombe Proteins/genetics/metabolism Histones/genetics/metabolism Gene Silencing Ccr4-Not complex H3K9me3 propagation heterochromatic gene silencing jumonji pr;
Notes:		"MedlineChallal, Drice Menant, Alexandra Goksal, Can Leroy, Estelle Al-Sady, Bassem Rougemaille, Mathieu eng R35 GM141888/GM/NIGMS NIH HHS/ P30 DK063720/DK/NIDDK NIH HHS/ Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. 2023/06/07 Genetics. 2023 Aug 9; 224(4):iyad108. doi: 10.1093/genetics/iyad108"