| Title: | The roots of plant defenses: integrative multivariate analyses uncover dynamic behaviors of gene and metabolic networks of roots elicited by leaf herbivory |
| Author(s): | Gulati J; Baldwin IT; Gaquerel E; |
| Address: | "Department of Molecular Ecology, Max Planck Institute for Chemical Ecology, Hans-Knoell-Strasse 8, 07745, Jena, Germany" |
| ISSN/ISBN: | 1365-313X (Electronic) 0960-7412 (Print) 0960-7412 (Linking) |
| Abstract: | "High-throughput analyses have frequently been used to characterize herbivory-induced reconfigurations in plant primary and secondary metabolism in above- and below-ground tissues, but the conclusions drawn from these analyses are often limited by the univariate methods used to analyze the data. Here we use our previously described multivariate time-series data analysis to evaluate leaf herbivory-elicited transcriptional and metabolic dynamics in the roots of Nicotiana attenuata. We observed large, but transient, systemic responses in the roots that contrasted with the pattern of co-linearity observed in the up- and downregulation of genes and metabolites across the entire time series in treated and systemic leaves. Using this newly developed approach for the analysis of whole-plant molecular responses in a time-course multivariate data set, we simultaneously analyzed stress responses in leaves and roots in response to the elicitation of a leaf. We found that transient systemic responses in roots resolved into two principal trends characterized by: (i) an inversion of root-specific semi-diurnal (12 h) transcript oscillations and (ii) transcriptional changes with major amplitude effects that translated into a distinct suite of root-specific secondary metabolites (e.g. alkaloids synthesized in the roots of N. attenuata). These findings underscore the importance of understanding tissue-specific stress responses in the correct day-night phase context and provide a holistic framework for the important role played by roots in above-ground stress responses" |
| Keywords: | "Animals Down-Regulation Gene Expression Profiling Gene Expression Regulation, Plant/*physiology Herbivory Manduca/physiology Metabolic Networks and Pathways *Metabolome Metabolomics Multivariate Analysis Organ Specificity Oxylipins/metabolism Plant Leaves;" |
| Notes: | "MedlineGulati, Jyotasana Baldwin, Ian T Gaquerel, Emmanuel eng 293926/ERC_/European Research Council/International Research Support, Non-U.S. Gov't England 2014/01/25 Plant J. 2014 Mar; 77(6):880-92. doi: 10.1111/tpj.12439. Epub 2014 Mar 8" |
