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Integr Zool

Title:		Role of the ejaculatory bulb in biosynthesis of the male pheromone cis-vaccenyl acetate in Drosophila melanogaster
Author(s):		Guiraudie-Capraz G; Pho DB; Jallon JM;
Address:		"Unite Mixte de Recherche Universite de Paris-Sud et Centre National de la Recherche Scientifique no8620, University of Paris Sud, Orsay, France"
Journal Title:		Integr Zool
Year:		2007
Volume:		2
Issue:		2
Page Number:		89 - 99
DOI:		10.1111/j.1749-4877.2007.00047.x
ISSN/ISBN:		1749-4869 (Print) 1749-4869 (Linking)
Abstract:		"In Drosophila melanogaster, the male ejaculatory bulb is the site of synthesis of a male-specific pheromone, cis-vaccenyl acetate, which functions as both an attractant and an anti-aphrodisiac. This long monounsaturated acetate is structurally similar to a number of shorter gland-synthesized moth pheromones. The cell monolayer that forms the Drosophila male ejaculatory bulb wall is responsible for the production and secretion of cis-vaccenyl acetate into the seminal fluid. When dissected bulbs were incubated with sodium [14-C]-acetate (or deuterated acetate), a labeled acetate ester was synthesized. The labeled acetate ester co-migrated with cis-vaccenyl acetate in thin layer chromatography. Incubation of the abdomens of males from which the ejaculatory bulbs had been removed, or the abdomens of females, with radiolabeled acetate did not yield any acetate ester, but did yield other lipid products, including hydrocarbons. When the isolated labeled acetate ester was hydrolyzed, no radioactive vaccenol was formed. This strongly suggests that the acetyl group is incorporated via a transacetylation reaction, but that the vaccenyl moiety is not synthetized in the blub. The transacetylation enzyme activity was localized in the microsomal subfraction of the bulb homogenate, and its affinity for vaccenol was not very different from that reported for monounsaturated alcohol substrates in moths"
Keywords:
Notes:		"PubMed-not-MEDLINEGuiraudie-Capraz, Gaelle Pho, Dang Ba Jallon, Jean-Marc eng Australia 2007/06/01 Integr Zool. 2007 Jun; 2(2):89-99. doi: 10.1111/j.1749-4877.2007.00047.x"