| Title: | Tuning Beforehand: A Foresight on RNA Interference (RNAi) and In Vitro-Derived dsRNAs to Enhance Crop Resilience to Biotic and Abiotic Stresses |
| Author(s): | Abdellatef E; Kamal NM; Tsujimoto H; |
| Address: | "Commission for Biotechnology and Genetic Engineering, National Center for Research, P.O. Box 2404, Khartoum 11111, Sudan. Arid Land Research Center, Tottori University, 1390 Hamasaka, Tottori 680-0001, Japan. Behavioural and Chemical Ecology Unit, International Centre of Insect Physiology and Ecology, P.O. Box 30772, Nairobi 00100, Kenya. Agricultural Research Corporation, P.O. Box 30, Khartoum North 11111, Sudan" |
| ISSN/ISBN: | 1422-0067 (Electronic) 1422-0067 (Linking) |
| Abstract: | "Crop yield is severely affected by biotic and abiotic stresses. Plants adapt to these stresses mainly through gene expression reprogramming at the transcriptional and post-transcriptional levels. Recently, the exogenous application of double-stranded RNAs (dsRNAs) and RNA interference (RNAi) technology has emerged as a sustainable and publicly acceptable alternative to genetic transformation, hence, small RNAs (micro-RNAs and small interfering RNAs) have an important role in combating biotic and abiotic stresses in plants. RNAi limits the transcript level by either suppressing transcription (transcriptional gene silencing) or activating sequence-specific RNA degradation (post-transcriptional gene silencing). Using RNAi tools and their respective targets in abiotic stress responses in many crops is well documented. Many miRNAs families are reported in plant tolerance response or adaptation to drought, salinity, and temperature stresses. In biotic stress, the spray-induced gene silencing (SIGS) provides an intelligent method of using dsRNA as a trigger to silence target genes in pests and pathogens without producing side effects such as those caused by chemical pesticides. In this review, we focus on the potential of SIGS as the most recent application of RNAi in agriculture and point out the trends, challenges, and risks of production technologies. Additionally, we provide insights into the potential applications of exogenous RNAi against biotic stresses. We also review the current status of RNAi/miRNA tools and their respective targets on abiotic stress and the most common responsive miRNA families triggered by stress conditions in different crop species" |
| Keywords: | "Animals Crop Production/methods Crop Protection/methods Crops, Agricultural/*genetics Gene Silencing Insect Control Insecta/genetics/pathogenicity MicroRNAs/genetics Plant Defense Against Herbivory/genetics *RNA Interference RNA, Double-Stranded/genetics;" |
| Notes: | "MedlineAbdellatef, Eltayb Kamal, Nasrein Mohamed Tsujimoto, Hisashi eng Postdoctoral Fellowships in Sub-Saharan Africa/Deutscher Akademischer Austauschdienst/ 19G21/Matsumae International Foundation/ JPMJSA1805/Japan Science and Technology Corporation/ Marginal Region Agriculture Project/Tottori University/ Review Switzerland 2021/07/25 Int J Mol Sci. 2021 Jul 19; 22(14):7687. doi: 10.3390/ijms22147687" |
