| Title: | Comparison of the in vitro anthelmintic effects of Acacia nilotica and Acacia raddiana |
| Author(s): | Zabre G; Kabore A; Bayala B; Katiki LM; Costa-Junior LM; Tamboura HH; Belem AMG; Abdalla AL; Niderkorn V; Hoste H; Louvandini H; |
| Address: | "Laboratoire de Biologie et Sante Animales-DPA/INERA, 04 BP 8645 Ouagadougou 04, Burkina Faso - Universite de Ouagadougou / UFR-SVT, 03 BP 7021 Ouagadougou 03, Burkina Faso. Laboratoire de Biologie et Sante Animales-DPA/INERA, 04 BP 8645 Ouagadougou 04, Burkina Faso. Universite de Ouagadougou / UFR-SVT, 03 BP 7021 Ouagadougou 03, Burkina Faso. Instituto de Zootecnia (SAA, APTA), Rua Heitor Penteado 56, Nova Odessa, SP, cep 13460-000, Brazil. Universidade Federal do Maranhao - UFMA, Campus do Bacanga CEP 65080-805 Sao Luis- MA, Brazil. Universite Nazi Boni, Bobo-Dioulasso, 01 BP 3770 Ouagadougou 01, Burkina Faso. Universidade de Sao Paulo, Centro de Energia Nuclear na Agricultura, NAPTSA, CP 96, CEP 13.400-970, Piracicaba, SP, Brazil. UMR1213 Herbivores, INRA - Clermont-Ferrand, Vetagro Sup, 63122 Saint-Genes-Champanelle, France. UMR IHAP 1225 INRA/ENVT, 23 Chemin des Capelles, 31076 Toulouse Cedex, France" |
| ISSN/ISBN: | 1776-1042 (Electronic) 1252-607X (Print) 1252-607X (Linking) |
| Abstract: | "Gastrointestinal nematodes are a major threat to small ruminant rearing in the Sahel area, where farmers traditionally use bioactive plants to control these worms, including Acacia nilotica and Acacia raddiana. The main aim of this study was to screen the potential anthelmintic properties of aqueous and acetone extracts of leaves of these two plants based on three in vitro assays: (1) the egg hatch inhibition assay (EHA); (2) the larvae exsheathment inhibition assay (LEIA) using Haemonchus contortus as a model; and (3) an adult mortality test (AMT) applied on Caenorhabditis elegans. For the EHA, only A. raddiana was effective with IC(50) = 1.58 mg/mL for aqueous extract, and IC(50) = 0.58 mg/mL for acetonic extract. For the LEIA, all extracts inhibited the exsheathment of larvae compared to the controls, and the aqueous extract of A. nilotica was more larvicidal with IC(50) = 0.195 mg/mL. In general, all responses to the substances were dose-dependent and were significantly different from the control group (p < 0.05). For the AMT, the extracts of the two Acacia species were effective but A. raddiana showed greater efficacy with 100% mortality at 2.5 mg/mL and LC(50) = 0.84 mg/mL (acetonic extract). The addition of polyvinyl polypyrrolidone (PVPP) to the extracts suggested that tannins were responsible for blocking egg eclosion and inducing adult mortality but were not responsible for exsheathment inhibition. These results suggest that the leaves of these Acacia species possess ovicidal and larvicidal activities in vitro against H. contortus, and adulticidal effects against C. elegans" |
| Keywords: | Acacia/*chemistry Animals Anthelmintics/isolation & purification/*pharmacology Caenorhabditis elegans/drug effects Feces/parasitology Goat Diseases/*drug therapy/parasitology Goats Haemonchiasis/drug therapy/veterinary Haemonchus/drug effects Helminthiasi; |
| Notes: | "MedlineZabre, Genevieve Kabore, Adama Bayala, Bale Katiki, Luciana M Costa-Junior, Livio Martins Tamboura, Hamidou H Belem, Adrien M G Abdalla, Adibe L Niderkorn, Vincent Hoste, Herve Louvandini, Helder eng Comparative Study France 2017/11/28 Parasite. 2017; 24:44. doi: 10.1051/parasite/2017044. Epub 2017 Nov 27" |
