| Title: | Characterizing potential repelling volatiles for 'push-pull' strategy against stem borer: a case study in Chilo auricilius |
| Author(s): | Yi X; Shi S; Wang P; Chen Y; Lu Q; Wang T; Zhou X; Zhong G; |
| Address: | "Key Laboratory of Crop Integrated Pest Management in South China, Ministry of Agriculture, South China Agricultural University, Guangzhou, China. Key Laboratory of Natural Pesticide and Chemical Biology, Ministry of Education, South China Agricultural University, Guangzhou, 510642, China. College of Agriculture, South China Agricultural University, Guangzhou, China. Guangdong Province Key Laboratory of Microbial Signals and Disease Control, Integrative Microbiology Research Centre, College of Agriculture, South China Agricultural University, Guangzhou, China. xiaofan_zhou@scau.edu.cn. Key Laboratory of Crop Integrated Pest Management in South China, Ministry of Agriculture, South China Agricultural University, Guangzhou, China. guohuazhong@scau.edu.cn. Key Laboratory of Natural Pesticide and Chemical Biology, Ministry of Education, South China Agricultural University, Guangzhou, 510642, China. guohuazhong@scau.edu.cn" |
| DOI: | 10.1186/s12864-019-6112-4 |
| ISSN/ISBN: | 1471-2164 (Electronic) 1471-2164 (Linking) |
| Abstract: | "BACKGROUND: Massive techniques have been evaluated for developing different pest control methods to minimize fertilizer and pesticide inputs. As 'push-pull' strategy utilizes generally non-toxic chemicals to manipulate behaviors of insects, such strategy is considered to be environmentally friendly. 'Push-pull' strategy has been extraordinarily effective in controlling stem borers, and the identification of new 'pushing' or 'pull' components against stem borers could be significantly helpful. RESULTS: In this study, the results of field trapping assay and behavioral assay showed the larvae of C.auricilius, one kind of stem borers, could be deterred by rice plant under tilling stage, its main host crop. The profiles of volatiles were compared between rice plants under two different developmental stages, and alpha-pinene was identified as a key differential component. The repelling activity of alpha-pinene against C.auricilius was confirmed by Y-tube olfactometer. For illuminating the olfactory recognition mechanism, transcriptome analysis was carried out, and 13 chemosensory proteins (CSPs) were identified in larvae and 19 CSPs were identified in adult of C.auriciliu, which was reported for the first time in this insect. Among these identified CSPs, 4 CSPs were significantly regulated by alpha-pinene treatment, and CSP8 showed good binding affinity with alpha-pinene in vitro. CONCLUSIONS: Overall, C.auricilius could be repelled by rice plant at tilling stage, and our results highlighted alpha-pinene as a key component in inducing repelling activity at this specific stage and confirmed the roles of some candidate chemosensory elements in this chemo-sensing process. The results in this study could provide valuable information for chemosensory mechanism of C.auricilius and for identification of 'push' agent against rice stem borers" |
| Keywords: | Animals Bicyclic Monoterpenes/metabolism Chemotaxis/genetics/physiology Gene Expression Profiling Gene Expression Regulation Insect Proteins/genetics/metabolism Insect Repellents/*metabolism Larva/genetics/physiology Moths/classification/genetics/*physiol; |
| Notes: | "MedlineYi, Xin Shi, Song Wang, Peidan Chen, Yaoyao Lu, Qiqi Wang, Tianyi Zhou, Xiaofan Zhong, Guohua eng 31701812/National Natural Science Foundation of China/ 2016LM1127/Special expert on pest control of modern agricultural industry technology system on economic grain and oil crop industry of Guangdong Province/ England 2019/10/19 BMC Genomics. 2019 Oct 17; 20(1):751. doi: 10.1186/s12864-019-6112-4" |
