| Title: | MAPK specificity in the yeast pheromone response independent of transcriptional activation |
| Author(s): | Breitkreutz A; Boucher L; Tyers M; |
| Address: | "Department of Medical Genetics and Microbiology, University of Toronto, 1 Kings College Circle, Toronto M5S 1A8, Canada" |
| DOI: | 10.1016/s0960-9822(01)00370-0 |
| ISSN/ISBN: | 0960-9822 (Print) 0960-9822 (Linking) |
| Abstract: | "The mechanisms whereby different external cues stimulate the same mitogen-activated protein kinase (MAPK) cascade, yet trigger an appropriately distinct biological response, epitomize the conundrum of specificity in cell signaling. In yeast, shared upstream components of the mating pheromone and filamentous growth pathways activate two related MAPKs, Fus3 and Kss1, which in turn regulate programs of gene expression via the transcription factor Ste12. As fus3, but not kss1, strains are impaired for mating, Fus3 exhibits specificity for the pheromone response. To account for this specificity, it has been suggested that Fus3 physically occludes Kss1 from pheromone-activated signaling complexes, which are formed on the scaffold protein Ste5. However, we find that genome-wide expression profiles of pheromone-treated wild-type, fus3, and kss1 deletion strains are highly correlated for all induced genes and, further, that two catalytically inactive versions of Fus3 fail to abrogate the pheromone-induced transcriptional response. Consistently, Fus3 and Kss1 kinase activity is induced to an equivalent extent in pheromone-treated cells. In contrast, both in vivo and in an in vitro-reconstituted MAPK system, Fus3, but not Kss1, exhibits strong substrate selectivity toward Far1, a bifunctional protein required for polarization and G(1) arrest. This effect accounts for the failure to repress G(1)-S specific transcription in fus3 strains and, in part, explains the mating defect of such strains. MAPK specificity in the pheromone response evidently occurs primarily at the substrate level, as opposed to specific kinase activation by dedicated signaling complexes" |
| Keywords: | "*Cell Cycle Proteins Cyclin-Dependent Kinase Inhibitor Proteins Enzyme Inhibitors/metabolism Fungal Proteins/genetics/*metabolism MAP Kinase Signaling System/*physiology Mitogen-Activated Protein Kinases/genetics/*metabolism Models, Biological Oligonucleo;" |
| Notes: | "MedlineBreitkreutz, A Boucher, L Tyers, M eng Research Support, Non-U.S. Gov't England 2001/08/30 Curr Biol. 2001 Aug 21; 11(16):1266-71. doi: 10.1016/s0960-9822(01)00370-0" |
