| Title: | Exploring the Terminal Pathway of Sex Pheromone Biosynthesis and Metabolism in the Silkworm |
| Author(s): | Wang QH; Gao X; Yu HS; Zhang Z; Yu QY; |
| Address: | "School of Life Sciences, Chongqing University, Chongqing 400044, China. School of Basic Medical Sciences, Zunyi Medical University, Zunyi 563000, China" |
| ISSN/ISBN: | 2075-4450 (Print) 2075-4450 (Electronic) 2075-4450 (Linking) |
| Abstract: | "Sex pheromones are vital to sexual communication and reproduction in insects. Although some key enzymes in pheromone production have been well studied, information on genes involved in the terminal pathway is limited. The domestic silkworm employs a pheromone blend containing (E,Z)-10,12-hexadecadienol (bombykol) and analogous (E,Z)-10,12-hexadecadienal (bombykal); whereas, its wild ancestor B. mandarina uses only bombykol. The two closely related moths might be a good model for exploring the genes involved in aldehyde pheromone synthesis and metabolism. By deep sequencing and analyzing the sex pheromone gland (PG) transcriptomes; we identified 116 candidate genes that may be related to pheromone biosynthesis, metabolism, and chemoreception. Spatiotemporal expression profiles and differentially expressed analysis revealed that four alcohol oxidases (BmorAO1; 2; 3; and 4); one aldehyde reductase (BmorAR1); and one aldehyde oxidase (BmorAOX5) might be involved in the terminal pathway. Phylogenetic analysis showed that, except for BmorAO3 and MsexAO3, AOs did not show a conversed orthologous relationship among moths; whereas, ARs and AOXs were phylogenetically conserved. This study provides crucial candidates for further functional elucidation, and which may be utilized as potential targets to disrupt sexual communication in other moth pests" |
| Keywords: | aldehyde synthesis and metabolism sex pheromone silkworm terminal pathway; |
| Notes: | "PubMed-not-MEDLINEWang, Qing-Hai Gao, Xing Yu, Hong-Song Zhang, Ze Yu, Quan-You eng U20A2058/National Natural Science Foundation of China/ cstc2020jcyj-msxmX0867/Natural Science Foundation Project of Chongqing/ cx2018066/Venture and Innovation Support Program for Chongqing Overseas Returnees/ 2020CDJ-LHZZ-038/Fundamental Research Funds for the Central Universities/ Switzerland 2021/12/24 Insects. 2021 Nov 26; 12(12):1062. doi: 10.3390/insects12121062" |
