| Title: | "Identification of genes involved in sex pheromone biosynthesis and metabolic pathway in the Chinese oak silkworm, Antheraea pernyi" |
| Author(s): | Wang QH; Gong Q; Fang SM; Liu YQ; Zhang Z; Yu QY; |
| Address: | "Laboratory of Evolutionary and Functional Genomics, School of Life Sciences, Chongqing University, Chongqing 401331, China. College of Life Science, China West Normal University, Nanchong 637002, China. Department of Sericulture, College of Bioscience and Biotechnology, Shenyang Agricultural University, 120 Dongling Road, Shenyang 110866, China. Laboratory of Evolutionary and Functional Genomics, School of Life Sciences, Chongqing University, Chongqing 401331, China. Electronic address: yuqy@cqu.edu.cn" |
| DOI: | 10.1016/j.ijbiomac.2020.07.263 |
| ISSN/ISBN: | 1879-0003 (Electronic) 0141-8130 (Linking) |
| Abstract: | "The Chinese oak silkworm, Antheraea pernyi, has not only been semi-domesticated as an important economical insect but also used for genetic research. The female moths of A. pernyi employ a pheromone blend containing (E,Z)-6,11-hexadecadienal (E6,Z11-16:Ald), (E,Z)-6,11-hexadecadienyl acetate (E6,Z11-16:OAc), and (E,Z)-4,9-tetradecadienyl acetate (E4,Z9-14:OAc). While its biosynthesis pathway is largely unknown. By deep sequencing and de novo assembly of sex pheromone gland (PG) transcriptome, we identified 141 candidate genes that are putatively related to pheromone biosynthesis, degradation, and chemoreception in A. pernyi. Gene expression patterns and phylogenetic analysis revealed that two desaturases (AperDES1 and 2), two fatty acid reductase (AperFAR1 and 2), and three acetyltransferase genes (AperACT1, 2 and 3) showed PG-biased or specific expression and were phylogenetically related to genes known to be involved in pheromone synthesis in other species. Furthermore, two carboxylesterases (AperCOE6 and 11) and two chemosensory protein (AperCSP1 and 6) were also expressed specifically or predominantly in the PGs, which might be related to sex pheromone degradation and transportation, respectively. Based on these results, the sex pheromone biosynthesis and metabolic pathway was proposed in A. pernyi. This study provides some crucial candidates for further functional elucidation, and may be used for interfering sexual communication in other Saturniidae pests" |
| Keywords: | Aldehyde Oxidoreductases/genetics Animals Biosynthetic Pathways/*genetics Bombyx/*genetics Female Insect Proteins/genetics Moths/*genetics Pheromones/*genetics Phylogeny Quercus Sex Attractants/*genetics Transcriptome/genetics Antheraea pernyi Biosynthesi; |
| Notes: | "MedlineWang, Qing-Hai Gong, Qian Fang, Shou-Min Liu, Yan-Qun Zhang, Ze Yu, Quan-You eng Netherlands 2020/08/07 Int J Biol Macromol. 2020 Nov 15; 163:1487-1497. doi: 10.1016/j.ijbiomac.2020.07.263. Epub 2020 Aug 2" |
