« Previous AbstractDetection of beta-alanyl aminopeptidase as a biomarker for Pseudomonas aeruginosa in the sputum of patients with cystic fibrosis using exogenous volatile organic compound evolution    Next AbstractChemosensory cues from the lacrimal and preputial glands stimulate production of IP3 in the vomeronasal organ and aggression in male mice »

Chem Senses

Title:		"Attenuation of the production of inositol 1,4,5-trisphosphate in the mouse vomeronasal organ by antibodies against the alphaq/11 subfamily of G-proteins"
Author(s):		Thompson RN; Napier A; Wekesa KS;
Address:		"Department of Biological Sciences, Alabama State University, Montgomery, 36101-0271, USA"
Journal Title:		Chem Senses
Year:		2006
Volume:		20060606
Issue:		7
Page Number:		613 - 619
DOI:		10.1093/chemse/bjj066
ISSN/ISBN:		0379-864X (Print) 0379-864X (Linking)
Abstract:		"The social and reproductive behaviors of most mammals are modulated by pheromones, which are perceived by the vomeronasal organ (VNO). Vomeronasal transduction in vertebrates is activated through G-protein-coupled receptors, which in turn leads to the generation of inositol 1,4,5-trisphosphate (IP(3)) and diacylglycerol (DAG) by the activity of phospholipase C. DAG has been shown to gate the transient receptor potential channel 2, whereas IP(3) may play a role in stimulating the release of calcium from the endoplasmic reticulum store. To investigate the role of the alpha subunits of G(q/11) in the transduction process, microvillar membranes from female mice VNO were preincubated with a selective C-terminal peptide antibody against Galpha(q/11) and then stimulated with adult male urine. Incubation of VNO membranes with antibodies against Galpha(q/11) blocked the production of IP(3) in a dose-dependent manner. We were also able to impair the production of IP(3) when we stimulated with 2-heptanone or 2,5-dimethylpyrazine in the presence of antibodies against the alpha subunit of G(q/11). 2-Heptanone is a known pheromone that has been linked to VIR receptors. Thus, our observations indicate that the alpha subunits of G(q/11) play a role in pheromonal signaling in the VNO"
Keywords:		"Angiotensin II/antagonists & inhibitors/pharmacology Animals Antibodies/*pharmacology Cell Membrane/chemistry/drug effects/metabolism Diglycerides/biosynthesis Dose-Response Relationship, Drug Enzyme Activation Female GTP-Binding Protein alpha Subunits, G;"
Notes:		"MedlineThompson, Roger N Napier, Audrey Wekesa, Kennedy S eng GM08219/GM/NIGMS NIH HHS/ P20 MD000547/MD/NIMHD NIH HHS/ Comparative Study Research Support, N.I.H., Extramural England 2006/06/08 Chem Senses. 2006 Sep; 31(7):613-9. doi: 10.1093/chemse/bjj066. Epub 2006 Jun 6"