« Previous AbstractInsect olfactory neurons in vitro: morphological and immunocytochemical characterization of male-specific antennal receptor cells from developing antennae of male Manduca sexta    Next AbstractInositol-trisphosphate-dependent calcium currents precede cation currents in insect olfactory receptor neurons in vitro »

J Exp Biol

Title:		Intracellular-messenger-mediated cation channels in cultured olfactory receptor neurons
Author(s):		Stengl M;
Address:		"Fakultat fur Biologie, Universitat Konstanz, Germany"
Journal Title:		J Exp Biol
Year:		1993
Volume:		178
Issue:
Page Number:		125 - 147
DOI:		10.1242/jeb.178.1.125
ISSN/ISBN:		0022-0949 (Print) 0022-0949 (Linking)
Abstract:		"After 2-3 weeks in culture, pupal olfactory receptor neurons from the antennae of male Manduca sexta respond to their species-specific sex pheromone by opening cation channels. These pheromone-dependent cation channels are the only channels previously found in cultured olfactory neurons that promote inward currents at membrane potentials more negative than the resting potential. The pheromone-dependent currents depend on external Ca2+ concentration. They are inwardly rectified with 10(-7) mol l-1 external Ca2+ and linear with 6 mmol l-1 external Ca2+. This paper shows that perfusion of cultured olfactory receptor neurons with GTP gamma S, ATP, inositol 1,4,5-trisphosphate or 10(-6) mol l-1 Ca2+ elicits cation currents resembling the pheromone-dependent cation currents in expressing inward rectification with 10(-7) mol l-1 external Ca2+ and being linear at external Ca2+ concentrations of 2 mumol l-1 or more. Stimulation with protein kinase C also elicits cation currents that share properties with the pheromone-dependent cation currents. All agent-induced cation currents appear to depend either directly or indirectly on Ca2+ concentration"
Keywords:		"Adenosine Triphosphate/pharmacology Animals Calcium/pharmacology Cations Cells, Cultured Electric Conductivity Guanosine 5'-O-(3-Thiotriphosphate)/pharmacology Inositol 1, 4, 5-Trisphosphate/pharmacology Ion Channels/*physiology Male *Moths Neurons/*physiol;"
Notes:		"MedlineStengl, M eng AI-23253/AI/NIAID NIH HHS/ Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. England 1993/05/01 J Exp Biol. 1993 May; 178:125-47. doi: 10.1242/jeb.178.1.125"