| Title: | alpha-Amylase Mediates Host Acceptance in the Braconid Parasitoid Cotesia flavipes |
| Author(s): | Bichang'a G; Da Lage JL; Capdevielle-Dulac C; Zivy M; Balliau T; Sambai K; Le Ru B; Kaiser L; Juma G; Maina ENM; Calatayud PA; |
| Address: | "IRD c/o icipe, P.O. Box 30772-00100, Nairobi, Kenya. Department of Biochemistry, University of Nairobi, P.O. Box 30197, Nairobi, Kenya. UMR Laboratoire Evolution, Genomes, Comportement et Ecologie, UPR 9034, 22 CNRS/IRD, 91198, Gif-sur-Yvette, France. Universite de Paris-Sud, 91405, Orsay, France. PAPPSO, GQE - Le Moulon, INRA, Univ. Paris-Sud, CNRS, AgroParisTech, Universite Paris-Saclay, 91190, Gif-sur-Yvette, France. IRD c/o icipe, P.O. Box 30772-00100, Nairobi, Kenya. pcalatayud@icipe.org. UMR Laboratoire Evolution, Genomes, Comportement et Ecologie, UPR 9034, 22 CNRS/IRD, 91198, Gif-sur-Yvette, France. pcalatayud@icipe.org. Universite de Paris-Sud, 91405, Orsay, France. pcalatayud@icipe.org" |
| DOI: | 10.1007/s10886-018-1002-9 |
| ISSN/ISBN: | 1573-1561 (Electronic) 0098-0331 (Linking) |
| Abstract: | "Foraging parasitoids use chemical signals in host recognition and selection processes. Although, the volatiles play a relevant role in the localization by parasitoids of their hosts feeding on plants, the host identification process for acceptance occurs mainly during contact between the parasitoid and its host where host products related to feeding activities, fecal pellets and oral secretions, play a crucial role. The purpose of this study was to identify the nature of the contact kairomone(s) that mediate the acceptance for oviposition of the parasitoid Cotesia flavipes Cameron (Hymenoptera, Braconidae), which was released in Kenya in 1993 to control the invasive crambid Chilo partellus (Swinhoe). Using host and non-hosts of C. flavipes, we showed that it is mainly the oral secretions of the larvae that harbour the active compound(s) that mediate host acceptance for oviposition by C. flavipes. Using an integration of behavioral observations and biochemical approaches, the active compound of the oral secretions was identified as an alpha-amylase. Using synthetized alpha-amylases from Drosophila melanogaster (an insect model for which syntheses of active and inactive alpha-amylases are available), we observed that the conformation of the enzyme rather than its catalytic site as well as its substrate and its degradation product is responsible for host acceptance and oviposition mediation of C. flavipes females. The results suggest that the alpha-amylase from oral secretions of the caterpillar host is a good candidate for an evolutionary solution to host acceptance for oviposition in C. flavipes" |
| Keywords: | "Animals Arthropod Antennae/drug effects/physiology Chromatography, High Pressure Liquid Electrophoresis, Polyacrylamide Gel Host-Parasite Interactions Insect Proteins/analysis/metabolism Larva/drug effects/physiology Oviposition Tandem Mass Spectrometry W;" |
| Notes: | "MedlineBichang'a, Gladys Da Lage, Jean-Luc Capdevielle-Dulac, Claire Zivy, Michel Balliau, Thierry Sambai, Kevin Le Ru, Bruno Kaiser, Laure Juma, Gerald Maina, Esther Njoki Mwangi Calatayud, Paul-Andre eng ANR-12-ADAP-0001/Agence Nationale de la Recherche/ 91560009/German Academic Exchange Service (DAAD)/ 2018/08/08 J Chem Ecol. 2018 Nov; 44(11):1030-1039. doi: 10.1007/s10886-018-1002-9. Epub 2018 Aug 7" |
