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J Dairy Sci

Title:		Comparison of microbiota and volatile organic compounds in milk from different sheep breeds
Author(s):		Bicer Y; Telli AE; Sonmez G; Telli N; Ucar G;
Address:		"Department of Food Hygiene and Technology, Faculty of Veterinary Medicine, Selcuk University, 42130, Konya, Turkey. Electronic address: yusufbicer@selcuk.edu.tr. Department of Food Hygiene and Technology, Faculty of Veterinary Medicine, Selcuk University, 42130, Konya, Turkey. Department of Genetics, Faculty of Veterinary Medicine, Selcuk University, 42130, Konya, Turkey. Department of Food Processing, Konya Technical University Vocational School of Technical Sciences, 42250, Konya, Turkey"
Journal Title:		J Dairy Sci
Year:		2021
Volume:		20210927
Issue:		12
Page Number:		12303 - 12311
DOI:		10.3168/jds.2021-20911
ISSN/ISBN:		1525-3198 (Electronic) 0022-0302 (Linking)
Abstract:		"In this study, we compared the microbiota and volatile organic compounds (VOC) present in the milk obtained from 3 different sheep breeds, namely Merino, Lacaune, and Assaf. Udder milk was collected from 21 animals, 7 from each breed. Bacterial microflora was determined metagenomically by extracting the DNA from the milk and analyzing the V3-V4 region of the 16S rRNA gene. Headspace solid-phase microextraction gas chromatography-mass spectrometry method was used to analyze VOC. The metagenomic analysis revealed (for Merino, Lacaune, and Assaf milk, respectively) Firmicutes (66.32, 69.36, and 57.08%), Actinobacteria (19.09, 7.67, and 19.40%), Proteobacteria (13.76, 21.06, and 22.19%), and Bacteroidetes (0.84, 1.91, and 1.33%) phyla in the milk samples. Lactobacillus was highly abundant in the milk of 3 breeds (29.64, 43.50, and 18.70%). The genera constituting more than 2% of all bacteria in all groups were Jeotgalicoccus (7.19, 5.34, and 10.77%), Enterococcus (5.18, 9.78, and 3.64%), and Corynebacterium (4.08, 3.00, and 13.44%). A total of 32 different VOC were identified by headspace solid-phase microextration analysis with 9, 30, and 24 different compounds from Merino, Lacaune, and Assaf breeds, respectively. Although ketone was the most abundant compound in Merino milk (71.84%), hydrocarbons were the most detected in Lacaune and Assaf milk (37.18% and 55.42%, respectively). A positive correlation was found between acetone, which was detected at the highest level in all groups, with Salinicoccus, Alloiococcus, Psychrobacter, and Dietzia. In addition, a negative correlation was found between the Lactobacillus genus, detected at the highest level in all groups, with methyl cyclopentane, 3-methylheptane, octane, decane, 3,3-dimethyloctane, and dodecane. Thus, differences were observed in the bacterial microflora and VOC in the sheep milk from different breeds under different feeding and breeding conditions"
Keywords:		"Animals *Microbiota Milk/chemistry RNA, Ribosomal, 16S/genetics Sheep Sheep, Domestic *Volatile Organic Compounds/analysis 16S rRNA headspace solid-phase microextraction gas chromatography-mass spectrometry metagenomic analysis sheep milk volatile organic;"
Notes:		"MedlineBicer, Yusuf Telli, A Ezgi Sonmez, Gonca Telli, Nihat Ucar, Gurkan eng 2021/10/02 J Dairy Sci. 2021 Dec; 104(12):12303-12311. doi: 10.3168/jds.2021-20911. Epub 2021 Sep 27"