| Title: | "Development of a UPLC-ESI-MS/MS method to measure urinary metabolites of selected VOCs: Benzene, cyanide, furfural, furfuryl alcohol, 5-hydroxymethylfurfural, and N-methyl-2-pyrrolidone" |
| Author(s): | Bhandari D; McCarthy D; Biren C; Movassaghi C; Blount BC; De Jesus VR; |
| Address: | "Centers for Disease Control and Prevention, Division of Laboratory Sciences, Tobacco and Volatiles Branch, Atlanta, GA 30341, United States of America. Electronic address: dbhandari@cdc.gov. Centers for Disease Control and Prevention, Division of Laboratory Sciences, Tobacco and Volatiles Branch, Atlanta, GA 30341, United States of America" |
| Journal Title: | J Chromatogr B Analyt Technol Biomed Life Sci |
| DOI: | 10.1016/j.jchromb.2019.121746 |
| ISSN/ISBN: | 1873-376X (Electronic) 1570-0232 (Print) 1570-0232 (Linking) |
| Abstract: | "We report on the development of an ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method for simultaneously measuring eight biomarkers of volatile organic compound (VOC) exposure, with potential application to e-cigarette aerosol biomonitoring. Phenylmercapturic acid (PMA) and trans, trans-muconic acid (tt-MA) are metabolites of benzene; 2-aminothiazoline-4-carboxylic acid (ATCA) is a metabolite of cyanide; N-2-furoylglycine (N2FG) is a metabolite of furfural and furfuryl alcohol; 5-hydroxymethylfuroic acid (HMFA), 5-hydroxymethyl-2-furoylglycine (HMFG), and 2,5-furandicarboxylic acid (FDCA) are metabolites of 5-hydroxymethylfurfural; and 5-hydroxy-N-methylpyrrolidone (5HMP) is a metabolite of N-methyl-2-pyrrolidone. A pentafluorophenyl-modified silica column was used for chromatographic separation. The overall run time for the method is about 6?ª+min per sample injection. The method has low to sub-nanograms per milliliter sensitivity, linearity over 3 orders of magnitude, and precision and accuracy within 15%. The method was used to measure human urine samples. Results showed that people with known benzene exposure (daily cigarette smokers) had higher levels of tt-MA and PMA compared with non-smokers. The method is advantageous for high-throughput analysis of selected VOC metabolites in large-scale, population-based studies such as the National Health and Nutrition Examination Survey (NHANES). Quantifying these urinary biomarkers is important to public health efforts to understand human exposure to VOCs from various sources, including tobacco products and electronic nicotine delivery systems" |
| Keywords: | "Benzene/*analysis/metabolism Chromatography, High Pressure Liquid/*methods Cyanides/metabolism/*urine Environmental Exposure/analysis Furaldehyde/analogs & derivatives/metabolism/*urine Humans Limit of Detection Linear Models Reproducibility of Results Sm;" |
| Notes: | "MedlineBhandari, Deepak McCarthy, Declan Biren, Chloe Movassaghi, Cameron Blount, Benjamin C De Jesus, Victor R eng CC999999/ImCDC/Intramural CDC HHS/ Netherlands 2019/08/28 J Chromatogr B Analyt Technol Biomed Life Sci. 2019 Sep 15; 1126-1127:121746. doi: 10.1016/j.jchromb.2019.121746. Epub 2019 Aug 14" |
