| Title: | Comparison of Switching and Biofilm Formation between MTL-Homozygous Strains of Candida albicans and Candida dubliniensis |
| Author(s): | Pujol C; Daniels KJ; Soll DR; |
| Address: | "The Developmental Studies Hybridoma Bank, Department of Biological Sciences, The University of Iowa, Iowa City, Iowa, USA. The Developmental Studies Hybridoma Bank, Department of Biological Sciences, The University of Iowa, Iowa City, Iowa, USA david-soll@uiowa.edu" |
| ISSN/ISBN: | 1535-9786 (Electronic) 1535-9778 (Print) 1535-9786 (Linking) |
| Abstract: | "Candida albicans and Candida dubliniensis are highly related species that share the same main developmental programs. In C. albicans, it has been demonstrated that the biofilms formed by strains heterozygous and homozygous at the mating type locus (MTL) differ functionally, but studies rarely identify the MTL configuration. This becomes a particular problem in studies of C. dubliniensis, given that one-third of natural strains are MTL homozygous. For that reason, we have analyzed MTL-homozygous strains of C. dubliniensis for their capacity to switch from white to opaque, the stability of the opaque phenotype, CO2 induction of switching, pheromone induction of adhesion, the effects of minority opaque cells on biofilm thickness and dry weight, and biofilm architecture in comparison with C. albicans. Our results reveal that C. dubliniensis strains switch to opaque at lower average frequencies, exhibit a far lower level of opaque phase stability, are not stimulated to switch by high CO2, exhibit more variability in biofilm architecture, and most notably, form mature biofilms composed predominately of pseudohyphae rather than true hyphae. Therefore, while several traits of MTL-homozygous strains of C. dubliniensis appear to be degenerating or have been lost, others, most notably several related to biofilm formation, have been conserved. Within this context, the possibility is considered that C. dubliniensis is transitioning from a hypha-dominated to a pseudohypha-dominated biofilm and that aspects of C. dubliniensis colonization may provide insights into the selective pressures that are involved" |
| Keywords: | "Adhesiveness/drug effects *Biofilms/drug effects Candida/cytology/drug effects/*genetics/*physiology Candida albicans/cytology/drug effects/*genetics/*physiology Carbon Dioxide/pharmacology *Genes, Mating Type, Fungal *Genes, Switch Heterozygote Homozygot;" |
| Notes: | "MedlinePujol, Claude Daniels, Karla J Soll, David R eng Comparative Study Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't 2015/10/04 Eukaryot Cell. 2015 Dec; 14(12):1186-202. doi: 10.1128/EC.00146-15. Epub 2015 Oct 2" |
