| Title: | The Consistency Between Phytotoxic Effects and the Dynamics of Allelochemicals Release from Eucalyptus globulus Leaves Used as Bioherbicide Green Manure |
| Author(s): | Puig CG; Goncalves RF; Valentao P; Andrade PB; Reigosa MJ; Pedrol N; |
| Address: | "Laboratory of Plant Ecophysiology, Department of Plant Biology and Soil Science, University of Vigo, Campus As Lagoas-Marcosende, 36310, Vigo, Spain. cgpuig@uvigo.es. REQUIMTE/LAQV, Laboratory of Pharmacognosy, Department of Chemistry, University of Porto, Rua de Jorge Viterbo Ferreira, 228, Porto, Portugal. Laboratory of Plant Ecophysiology, Department of Plant Biology and Soil Science, University of Vigo, Campus As Lagoas-Marcosende, 36310, Vigo, Spain" |
| DOI: | 10.1007/s10886-018-0983-8 |
| ISSN/ISBN: | 1573-1561 (Electronic) 0098-0331 (Linking) |
| Abstract: | "In the worldwide search for new strategies in sustainable weed management, the use of allelopathic plants incorporated into the soil as green manure can help control weeds by releasing allelochemicals into the environment. In previous experiments, Eucalyptus globulus leaves incorporated into the soil as green manure were shown to have a notable potential for weed control. But, 'what was exactly happening at chemical level?' and 'which were the compounds potentially responsible for the phytotoxic effects observed during those greenhouse assays?' In the present study, in-vitro phytotoxicity bioassays and chemical analysis of eucalyptus leaves were carried out in order to explore the relationship between the temporal phytotoxic effects and the dynamics of chemical composition. For that, eucalyptus leaves were removed from soil at different sampling times during 30 days and analyzed for phenolic and volatile organic compounds (VOCs) by HPLC and HS-SPME/GC-MS, respectively. The phytotoxic potential of the aqueous extract and the volatile fraction was tested on the germination and early growth of Lactuca sativa. Eucalyptus leaves incorporated into the soil as green manure showed a continuous release of different phenolic and volatile compounds during a 30-day period of decomposition. Both fractions had phytotoxic effects during the time assayed; however, the target process of phytotoxicity was different: phenolic compounds being the factor causing germination inhibition and VOCs responsible for growth reduction. The dynamics of release of this cocktail of allelochemicals into the soil environment may be the responsible for the phytotoxicity observed in our previous works" |
| Keywords: | Allelopathy Eucalyptus/chemistry/*physiology Germination/drug effects Lettuce/drug effects/growth & development Phenols/analysis/metabolism Pheromones/analysis/*metabolism/toxicity Plant Leaves/chemistry/*physiology Soil/chemistry Volatile Organic Compoun; |
| Notes: | "MedlinePuig, Carolina G Goncalves, Rui F Valentao, Patricia Andrade, Paula B Reigosa, Manuel J Pedrol, Nuria eng CGL2016-78660-R/Secretaria de Estado de Investigacion, Desarrollo e Innovacion/ AP2009-4809/Ministerio de Educacion, Cultura y Deporte/ 2018/07/25 J Chem Ecol. 2018 Aug; 44(7-8):658-670. doi: 10.1007/s10886-018-0983-8. Epub 2018 Jul 24" |
