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Biomolecules

Title:		The Mode of Action of Cyclic Monoterpenes (-)-Limoneneand (+)-alpha-Pinene on Bacterial Cells
Author(s):		Melkina OE; Plyuta VA; Khmel IA; Zavilgelsky GB;
Address:		"State Research Institute of Genetics and Selection of Industrial Microorganisms of the National Research Centre 'Kurchatov Institute', Kurchatov Genomic Center, 117545 Moscow, Russia. Institute of Molecular Genetics of the National Research Center 'Kurchatov Institute', 123182 Moscow, Russia"
Journal Title:		Biomolecules
Year:		2021
Volume:		20210529
Issue:		6
Page Number:		-
DOI:		10.3390/biom11060806
ISSN/ISBN:		2218-273X (Electronic) 2218-273X (Linking)
Abstract:		"A broad spectrum of volatile organic compounds' (VOCs') biological activities has attracted significant scientific interest, but their mechanisms of action remain little understood. The mechanism of action of two VOCs-the cyclic monoterpenes (-)-limonene and (+)-alpha-pinene-on bacteria was studied in this work. We used genetically engineered Escherichia coli bioluminescent strains harboring stress-responsive promoters (responsive to oxidative stress, DNA damage, SOS response, protein damage, heatshock, membrane damage) fused to the luxCDABE genes of Photorhabdus luminescens. We showed that (-)-limonene induces the PkatG and PsoxS promoters due to the formation of reactive oxygen species and, as a result, causes damage to DNA (SOSresponse), proteins (heat shock), and membrane (increases its permeability). The experimental data indicate that the action of (-)-limonene at high concentrations and prolonged incubation time makes degrading processes in cells irreversible. The effect of (+)-alpha-pinene is much weaker: it induces only heat shock in the bacteria. Moreover, we showed for the first time that (-)-limonene completely inhibits the DnaKJE-ClpB bichaperone-dependent refolding of heat-inactivated bacterial luciferase in both E. coli wild type and mutant DeltaibpB strains. (+)-alpha-Pinene partially inhibits refolding only in DeltaibpB mutant strain"
Keywords:		"*Bacterial Proteins/genetics/metabolism *Bicyclic Monoterpenes/chemistry/metabolism/pharmacology *DNA Damage *DNA, Bacterial/genetics/metabolism *Escherichia coli K12/genetics/metabolism *Limonene/chemistry/metabolism/pharmacology Photorhabdus/genetics SO;"
Notes:		"MedlineMelkina, Olga E Plyuta, Vladimir A Khmel, Inessa A Zavilgelsky, Gennadii B eng Research Support, Non-U.S. Gov't Switzerland 2021/06/03 Biomolecules. 2021 May 29; 11(6):806. doi: 10.3390/biom11060806"