| Title: | MAP kinases with distinct inhibitory functions impart signaling specificity during yeast differentiation |
| Author(s): | Madhani HD; Styles CA; Fink GR; |
| Address: | "Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142, USA" |
| DOI: | 10.1016/s0092-8674(00)80454-7 |
| ISSN/ISBN: | 0092-8674 (Print) 0092-8674 (Linking) |
| Abstract: | "Filamentous invasive growth of S. cerevisiae requires multiple elements of the mitogen-activated protein kinase (MAPK) signaling cascade that are also components of the mating pheromone response pathway. Here we show that, despite sharing several constituents, the two pathways use different MAP kinases. The Fus3 MAPK regulates mating, whereas the Kss1 MAPK regulates filamentation and invasion. Remarkably, in addition to their kinase-dependent activation functions, Kss1 and Fus3 each have a distinct kinase-independent inhibitory function. Kss1 inhibits the filamentation pathway by interacting with its target transcription factor Ste12. Fus3 has a different inhibitory activity that prevents the inappropriate activation of invasion by the pheromone response pathway. In the absence of Fus3, there is erroneous crosstalk in which mating pheromone now activates filamentation-specific gene expression using the Kss1 MAPK" |
| Keywords: | "Amino Acids/metabolism Calcium-Calmodulin-Dependent Protein Kinases/*metabolism Fungal Proteins/genetics/metabolism Genes, Fungal/physiology Haploidy Mating Factor Mitogen-Activated Protein Kinase Kinases *Mitogen-Activated Protein Kinases Mutation Peptid;" |
| Notes: | "MedlineMadhani, H D Styles, C A Fink, G R eng Research Support, Non-U.S. Gov't 1997/12/11 Cell. 1997 Nov 28; 91(5):673-84. doi: 10.1016/s0092-8674(00)80454-7" |
