| Title: | Effect of fungal indoor air pollutant 1-octen-3-ol on levels of reactive oxygen species and nitric oxide as well as dehydrogenases activities in drosophila melanogaster males |
| Author(s): | Macedo GE; Vieira PB; Rodrigues NR; Gomes KK; Rodrigues JF; Franco JL; Posser T; |
| Address: | "Oxidative Stress and Cell Signaling Research Group, Centro Interdisciplinar em Biotecnologia - CIPBIOTEC, Universidade Federal do Pampa, Campus Sao Gabriel, Sao Gabriel, Brazil. Departamento de Bioquimica e Biologia Molecular, CCNE, Universidade Federal de Santa Maria, Santa Maria, Brazil" |
| Journal Title: | J Toxicol Environ Health A |
| DOI: | 10.1080/15287394.2022.2054887 |
| ISSN/ISBN: | 1528-7394 (Print) 0098-4108 (Linking) |
| Abstract: | "Fungal pollution of indoor environments contributes to several allergic symptoms and represents a public health problem. It is well-established that 1-octen-3-ol, also known as mushroom alcohol, is a fungal volatile organic compound (VOC) commonly found in damp indoor spaces and responsible for the typical musty odor. Previously it was reported that exposure to 1-octen-3-ol induced inflammations and disrupted mitochondrial morphology and bioenergetic rate in Drosophila melanogaster. The aim of this study was to examine the influence of 1-octen-3-ol on dehydrogenase activity, apoptotic biomarkers, levels of nitric oxide (NO) and reactive oxygen species (ROS), as well as antioxidant enzymes activities. D. melanogaster flies were exposed to an atmosphere containing 1-octen-3-ol (2.5 or infinityl/L) for 24 hr. Data demonstrated that 1-octen-3-ol decreased dehydrogenases activity and NO levels but increased ROS levels accompanied by stimulation of glutathione-S-transferase (GST) and superoxide dismutase (SOD) activities without altering caspase 3/7 activation. These findings indicate that adverse mitochondrial activity effects following exposure of D. melanogaster to 1-octen-3-ol, a fungal VOC, may be attributed to oxidant stress. The underlying mechanisms involved in adverse consequences of indoor fungal exposure appear to be related to necrotic but not apoptotic mechanisms. The adverse consequences were sex-dependent with males displaying higher sensitivity to 1-octen-3-ol. Based upon on the fact that the fly genome shares nearly 75% of disease-related genes to human exposure to this fungus may explain the adverse human responses to mold especially for males" |
| Keywords: | *Air Pollutants Animals Antioxidants/pharmacology Drosophila melanogaster Male Nitric Oxide Octanols Oxidoreductases Reactive Oxygen Species *Volatile Organic Compounds/analysis/toxicity 1-octen-3-ol mold risk assessment toxicity volatile organic compound; |
| Notes: | "MedlineMacedo, Giulianna Echeverria Vieira, Patricia de Brum Rodrigues, Nathane Rosa Gomes, Karen Kich Rodrigues, Jessica Ferreira Franco, Jeferson Luis Posser, Thais eng Research Support, Non-U.S. Gov't England 2022/04/01 J Toxicol Environ Health A. 2022 Jul 18; 85(14):573-585. doi: 10.1080/15287394.2022.2054887. Epub 2022 Mar 31" |
