| Title: | Fungal compound 1-octen-3-ol induces mitochondrial morphological alterations and respiration dysfunctions in Drosophila melanogaster |
| Author(s): | Macedo GE; de Brum Vieira P; Rodrigues NR; Gomes KK; Martins IK; Franco JL; Posser T; |
| Address: | "Oxidative Stress and Cell Signaling Research Group, Centro Interdisciplinar Em Biotecnologia - CIPBIOTEC, Universidade Federal Do Pampa, Campus Sao Gabriel, 97307-020, Sao Gabriel, RS, Brazil. Electronic address: giulianna.echeverria@gmail.com. Oxidative Stress and Cell Signaling Research Group, Centro Interdisciplinar Em Biotecnologia - CIPBIOTEC, Universidade Federal Do Pampa, Campus Sao Gabriel, 97307-020, Sao Gabriel, RS, Brazil. Electronic address: patriciasbrum@yahoo.com. Oxidative Stress and Cell Signaling Research Group, Centro Interdisciplinar Em Biotecnologia - CIPBIOTEC, Universidade Federal Do Pampa, Campus Sao Gabriel, 97307-020, Sao Gabriel, RS, Brazil; Departamento de Bioquimica e Biologia Molecular, CCNE, Universidade Federal de Santa Maria, 97105-900, Santa Maria, RS, Brazil. Electronic address: nathane.r.rodrigues@gmail.com. Oxidative Stress and Cell Signaling Research Group, Centro Interdisciplinar Em Biotecnologia - CIPBIOTEC, Universidade Federal Do Pampa, Campus Sao Gabriel, 97307-020, Sao Gabriel, RS, Brazil. Electronic address: karenkich.bio@gmail.com. Oxidative Stress and Cell Signaling Research Group, Centro Interdisciplinar Em Biotecnologia - CIPBIOTEC, Universidade Federal Do Pampa, Campus Sao Gabriel, 97307-020, Sao Gabriel, RS, Brazil. Electronic address: illanakemmerich@gmail.com. Oxidative Stress and Cell Signaling Research Group, Centro Interdisciplinar Em Biotecnologia - CIPBIOTEC, Universidade Federal Do Pampa, Campus Sao Gabriel, 97307-020, Sao Gabriel, RS, Brazil; Departamento de Bioquimica e Biologia Molecular, CCNE, Universidade Federal de Santa Maria, 97105-900, Santa Maria, RS, Brazil. Electronic address: jefersonfranco@gmail.com. Oxidative Stress and Cell Signaling Research Group, Centro Interdisciplinar Em Biotecnologia - CIPBIOTEC, Universidade Federal Do Pampa, Campus Sao Gabriel, 97307-020, Sao Gabriel, RS, Brazil. Electronic address: thaisposser@unipampa.edu.br" |
| DOI: | 10.1016/j.ecoenv.2020.111232 |
| ISSN/ISBN: | 1090-2414 (Electronic) 0147-6513 (Linking) |
| Abstract: | "Fungal volatile organic compounds (VOCs) comprise a group of compounds commonly found in damp or water-damaged indoor places affecting air quality. Indoor fungal pollution is a severe threat to human health, contributing to the onset of allergic diseases. The compound 1-octen-3-ol, known as 'mushroom alcohol', is the most abundant VOC and confers the characteristic mold odor. Exposure to 1-octen-3-ol induces inflammatory markers and episodes of allergic rhinitis and conjunctivitis; however, the effects of this compound towards mitochondria are fairly known. The present study aimed to evaluate the effects of 1-octen-3-ol on inflammatory targets and on mitochondrial morphology and bioenergetic rate in D. melanogaster. Drosophilas were exposed by inhalation to 2.5 muL/L and 5 muL/L of 1-octen-3-ol for 24 h. Observation showed a decreasing in the survival and locomotor ability of flies. Superoxide dismutase (SOD) activity was induced whereas Catalase (CAT) activity was inhibited. Analysis of the mitochondria respiration, detected inhibition of complex I and II in the electron transport chain and a decreased bioenergetic rate. Electronic microscopy provided morphological insights of the mitochondrial status in which a disarrangement in mitochondrial cristae profile was observed. 1-Octen-3-ol induced increased activity of caspase 3/7 and ERK phosphorylation. The mRNA relative steady-state levels of p38(MAPK) and JNK were down-regulated, whereas NF-kappaB and p53 were up-regulated. In parallel, nitrite levels were induced in relation to the non-exposed group. These findings point to the mitochondria as a crucial target for the toxicity of 1-octen-3-ol in parallel with activation of pro-inflammatory factors and apoptotic signaling pathway cascade" |
| Keywords: | "Air Pollution Air Pollution, Indoor/adverse effects Animals Antioxidants/metabolism Apoptosis/drug effects Drosophila melanogaster/*drug effects/enzymology/genetics Female Fungi/metabolism Gene Expression/drug effects Humans Male Mitochondria/*drug effect;" |
| Notes: | "MedlineMacedo, Giulianna Echeverria de Brum Vieira, Patricia Rodrigues, Nathane Rosa Gomes, Karen Kich Martins, Illana Kemmerich Franco, Jeferson Luis Posser, Thais eng Netherlands 2020/09/06 Ecotoxicol Environ Saf. 2020 Dec 15; 206:111232. doi: 10.1016/j.ecoenv.2020.111232. Epub 2020 Sep 2" |
