| Title: | "Metabolic, Enzymatic Activity, and Transcriptomic Analysis Reveals the Mechanism Underlying the Lack of Characteristic Floral Scent in Apricot Mei Varieties" |
| Author(s): | Bao F; Zhang T; Ding A; Ding A; Yang W; Wang J; Cheng T; Zhang Q; |
| Address: | "Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing Forestry University, Beijing, China. Beijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding, National Engineering Research Center for Floriculture, Beijing Laboratory of Urban and Rural Ecological Environment, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education, School of Landscape Architecture, Beijing Forestry University, Beijing, China" |
| ISSN/ISBN: | 1664-462X (Print) 1664-462X (Electronic) 1664-462X (Linking) |
| Abstract: | "Apricot mei, a hybrid of Prunus mume and Prunus sibirica, usually has greater cold resistance than P. mume; however, most varieties of Apricot mei lack the characteristic floral scent of P. mume. The volatile and intracellular metabolites, activity levels of key enzymes, and transcriptomes of blooming flowers were comprehensively investigated in five varieties of P. mume. Benzyl acetate and eugenol were determined to be the main components of the P. mume floral scent. However, benzyl benzoate and benzyl alcohol benzoyltransferase activity was detected in only the low-fragrance varieties 'Dan Fenghou' and 'Yanxing.' No benzyl alcohol or benzaldehyde reductase (BAR) activity was detected in the non-fragrant variety 'Fenghou.' PmBAR1 and PmBAR3 were identified as the key genes responsible for BAR activity. The lack of benzyl alcohol synthesis in the 'Fenghou' variety was caused by low activity of PmBAR1-Fen and low expression of PmBAR3. The 60-aa segment at the N-terminus of PmBAR3 was found to play an important role in its enzymatic activity. Correlation tests between floral scent metabolites and the transcriptomes of the five different scented varieties showed that some transcripts associated with hormones, stresses, posttranslational modifications and transporters may also play important regulatory roles in floral scent metabolism in the different varieties" |
| Keywords: | PmBAR Prunus mume apricot mei benzyl acetate benzyl alcohol benzyl benzoate eugenol floral scent; |
| Notes: | "PubMed-not-MEDLINEBao, Fei Zhang, Tengxun Ding, Anqi Ding, Aiqin Yang, Weiru Wang, Jia Cheng, Tangren Zhang, Qixiang eng Switzerland 2020/11/17 Front Plant Sci. 2020 Oct 22; 11:574982. doi: 10.3389/fpls.2020.574982. eCollection 2020" |
