| Title: | Anti-Haemophilus Activity of Selected Essential Oils Detected by TLC-Direct Bioautography and Biofilm Inhibition |
| Author(s): | Balazs VL; Horvath B; Kerekes E; Acs K; Kocsis B; Varga A; Boszormenyi A; Nagy DU; Krisch J; Szechenyi A; Horvath G; |
| Address: | "Department of Pharmacognosy, Faculty of Pharmacy, University of Pecs, H-7624 Pecs, Hungary. balazsviktorialilla@gmail.com. Institute of Pharmaceutical Technology and Biopharmacy, Faculty of Pharmacy, University of Pecs, H-7624 Pecs, Hungary. bai0311@gmail.com. Department of Microbiology, Faculty of Science and Informatics, University of Szeged, H-6726 Szeged, Hungary. kerekeserika88@gmail.com. Department of Pharmacognosy, Faculty of Pharmacy, University of Pecs, H-7624 Pecs, Hungary. Department of Medical Microbiology and Immunology, Medical School, University of Pecs, H-7624 Pecs, Hungary. kocsis.bela@pte.hu. Department of Medical Microbiology and Immunology, Medical School, University of Pecs, H-7624 Pecs, Hungary. adorjanvarga@ymail.com. Institute of Pharmacognosy, Faculty of Pharmacy, Semmelweis University, H-1085 Budapest, Hungary. aboszormenyi@gmail.com. Department of Genetics and Molecular Biology, Institute of Biology, Faculty of Sciences, University of Pecs, H-7624 Pecs, Hungary. davenagy9@gmail.com. Department of Food Engineering, Faculty of Engineering, University of Szeged, H-6724 Szeged, Hungary. krisch@mk.u-szeged.hu. Institute of Pharmaceutical Technology and Biopharmacy, Faculty of Pharmacy, University of Pecs, H-7624 Pecs, Hungary. szealex@gamma.ttk.pte.hu. Department of Pharmacognosy, Faculty of Pharmacy, University of Pecs, H-7624 Pecs, Hungary. horvath.gyorgyi@gytk.pte.hu" |
| DOI: | 10.3390/molecules24183301 |
| ISSN/ISBN: | 1420-3049 (Electronic) 1420-3049 (Linking) |
| Abstract: | "Essential oils (EOs) are becoming increasingly popular in medical applications because of their antimicrobial effect. Direct bioautography (DB) combined with thin layer chromatography (TLC) is a screening method for the detection of antimicrobial compounds in plant extracts, for example, in EOs. Due to their lipophilic character, the common microbiological assays (etc. disk diffusion) could not provide reliable results. The aim of this study was the evaluation of antibacterial and anti-biofilm properties of the EO of cinnamon bark, clove, peppermint, thyme, and their main components against Haemophilus influenzae and H. parainfluenzae. Oil in water (O/W) type Pickering nano-emulsions stabilized with silica nanoparticles from each oil were prepared to increase their water-solubility. Samples with Tween80 surfactant and absolute ethanol were also used. Results showed that H. influenzae was more sensitive to the EOs than H. parainfluenzae (except for cinnamon bark oil). In thin layer chromatography-direct bioautography (TLC-DB) the ethanolic solutions of thyme oil presented the best activity against H. influenzae, while cinnamon oil was the most active against H. parainfluenzae. Pickering nano-emulsion of cinnamon oil inhibited the biofilm formation of H. parainfluenzae (76.35%) more efficiently than samples with Tween80 surfactant or absolute ethanol. In conclusion, Pickering nano-emulsion of EOs could inhibit the biofilm production effectively" |
| Keywords: | "Anti-Bacterial Agents/*pharmacology Biofilms/*drug effects Chromatography, Thin Layer/*methods Emulsions/chemistry Haemophilus/*drug effects/*physiology Microbial Sensitivity Tests Nanoparticles/chemistry Oils, Volatile/*pharmacology Volatile Organic Comp;" |
| Notes: | "MedlineBalazs, Viktoria Lilla Horvath, Barbara Kerekes, Erika Acs, Kamilla Kocsis, Bela Varga, Adorjan Boszormenyi, Andrea Nagy, David U Krisch, Judit Szechenyi, Aleksandar Horvath, Gyorgyi eng NKFI 18 K 128217/NKFI (National Research, Development and Innovation Office)/ Switzerland 2019/09/14 Molecules. 2019 Sep 11; 24(18):3301. doi: 10.3390/molecules24183301" |
