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J Cell Biol

Title:		A conserved protein interaction network involving the yeast MAP kinases Fus3 and Kss1
Author(s):		Kusari AB; Molina DM; Sabbagh W; Lau CS; Bardwell L;
Address:		"Dept. of Developmental and Cell Biology, 5205 McGaugh Hall, University of California, Irvine, Irvine, CA 92697-2300, USA"
Journal Title:		J Cell Biol
Year:		2004
Volume:		164
Issue:		2
Page Number:		267 - 277
DOI:		10.1083/jcb.200310021
ISSN/ISBN:		0021-9525 (Print) 1540-8140 (Electronic) 0021-9525 (Linking)
Abstract:		"The Saccharomyces cerevisiae mitogen-activated protein kinases (MAPKs) Fus3 and Kss1 bind to multiple regulators and substrates. We show that mutations in a conserved docking site in these MAPKs (the CD/7m region) disrupt binding to an important subset of their binding partners, including the Ste7 MAPK kinase, the Ste5 adaptor/scaffold protein, and the Dig1 and Dig2 transcriptional repressors. Supporting the possibility that Ste5 and Ste7 bind to the same region of the MAPKs, they partially competed for Fus3 binding. In vivo, some of the MAPK mutants displayed reduced Ste7-dependent phosphorylation, and all of them exhibited multiple defects in mating and pheromone response. The Kss1 mutants were also defective in Kss1-imposed repression of Ste12. We conclude that MAPKs contain a structurally and functionally conserved docking site that mediates an overall positively acting network of interactions with cognate docking sites on their regulators and substrates. Key features of this interaction network appear to have been conserved from yeast to humans"
Keywords:		"*Adaptor Proteins, Signal Transducing Amino Acid Sequence Binding Sites Carrier Proteins/metabolism Homeostasis Mitogen-Activated Protein Kinase Kinases Mitogen-Activated Protein Kinases/genetics/*metabolism Molecular Sequence Data Mutagenesis, Site-Direc;"
Notes:		"MedlineKusari, Anasua B Molina, Douglas M Sabbagh, Walid Jr Lau, Chang S Bardwell, Lee eng R33 GM069013/GM/NIGMS NIH HHS/ GM60366/GM/NIGMS NIH HHS/ GM69013/GM/NIGMS NIH HHS/ R01 GM060366/GM/NIGMS NIH HHS/ R21 GM069013/GM/NIGMS NIH HHS/ Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. 2004/01/22 J Cell Biol. 2004 Jan 19; 164(2):267-77. doi: 10.1083/jcb.200310021"