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Metabolites

Title:		Development of a Direct Headspace Collection Method from Arabidopsis Seedlings Using HS-SPME-GC-TOF-MS Analysis
Author(s):		Kusano M; Iizuka Y; Kobayashi M; Fukushima A; Saito K;
Address:		"RIKEN Plant Science Center, Tsurumi, Yokohama 230-0045, Japan. mkusano005@psc.riken.jp. RIKEN Plant Science Center, Tsurumi, Yokohama 230-0045, Japan. yiizuka-ri@psc.riken.jp. RIKEN Plant Science Center, Tsurumi, Yokohama 230-0045, Japan. kobamako@riken.jp. RIKEN Plant Science Center, Tsurumi, Yokohama 230-0045, Japan. a-fukush@psc.riken.jp. RIKEN Plant Science Center, Tsurumi, Yokohama 230-0045, Japan. ksaito@psc.riken.jp"
Journal Title:		Metabolites
Year:		2013
Volume:		20130409
Issue:		2
Page Number:		223 - 242
DOI:		10.3390/metabo3020223
ISSN/ISBN:		2218-1989 (Print) 2218-1989 (Electronic) 2218-1989 (Linking)
Abstract:		"Plants produce various volatile organic compounds (VOCs), which are thought to be a crucial factor in their interactions with harmful insects, plants and animals. Composition of VOCs may differ when plants are grown under different nutrient conditions, i.e., macronutrient-deficient conditions. However, in plants, relationships between macronutrient assimilation and VOC composition remain unclear. In order to identify the kinds of VOCs that can be emitted when plants are grown under various environmental conditions, we established a conventional method for VOC profiling in Arabidopsis thaliana (Arabidopsis) involving headspace-solid-phase microextraction-gas chromatography-time-of-flight-mass spectrometry (HS-SPME-GC-TOF-MS). We grew Arabidopsis seedlings in an HS vial to directly perform HS analysis. To maximize the analytical performance of VOCs, we optimized the extraction method and the analytical conditions of HP-SPME-GC-TOF-MS. Using the optimized method, we conducted VOC profiling of Arabidopsis seedlings, which were grown under two different nutrition conditions, nutrition-rich and nutrition-deficient conditions. The VOC profiles clearly showed a distinct pattern with respect to each condition. This study suggests that HS-SPME-GC-TOF-MS analysis has immense potential to detect changes in the levels of VOCs in not only Arabidopsis, but other plants grown under various environmental conditions"
Keywords:
Notes:		"PubMed-not-MEDLINEKusano, Miyako Iizuka, Yumiko Kobayashi, Makoto Fukushima, Atsushi Saito, Kazuki eng Switzerland 2013/01/01 Metabolites. 2013 Apr 9; 3(2):223-42. doi: 10.3390/metabo3020223"